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dc.contributor.author
You, Yuan
dc.contributor.author
Koczyk, Grzegorz
dc.contributor.author
Nuc, Maria
dc.contributor.author
Morbitzer, Robert
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Holmes, Danalyn R.
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von Roepenack Lahaye, Edda
dc.contributor.author
Hou, Shiji
dc.contributor.author
Giudicatti, Axel Joel
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dc.contributor.author
Gris, Carine
dc.contributor.author
Manavella, Pablo Andrés
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dc.contributor.author
Noël, Laurent D.
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Krajewski, Paweł
dc.contributor.author
Lahaye, Thomas
dc.date.available
2023-10-02T12:51:38Z
dc.date.issued
2022-12
dc.identifier.citation
You, Yuan; Koczyk, Grzegorz; Nuc, Maria; Morbitzer, Robert; Holmes, Danalyn R.; et al.; The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence; Springer; Nature Plants; 9; 1; 12-2022; 128-141
dc.identifier.issn
2055-0278
dc.identifier.uri
http://hdl.handle.net/11336/213731
dc.description.abstract
Bacteria inject effector proteins into host cells to manipulate cellular processes that promote disease. Since bacteria deliver minuscule amounts of effectors only into targeted host cells, it is technically challenging to capture effector-dependent cellular changes from bulk-infected host tissues. Here, we report a new technique called effector-inducible isolation of nuclei tagged in specific cell types (eINTACT), which facilitates affinity-based purification of nuclei from Arabidopsis plant cells that have received Xanthomonas bacterial effectors. Analysis of purified nuclei reveals that the Xanthomonas effector XopD manipulates the expression of Arabidopsis abscisic acid signalling-related genes and activates OSCA1.1, a gene encoding a calcium-permeable channel required for stomatal closure in response to osmotic stress. The loss of OSCA1.1 causes leaf wilting and reduced bacterial growth in infected leaves, suggesting that OSCA1.1 promotes host susceptibility. eINTACT allows us to uncover that XopD exploits host OSCA1.1/abscisic acid osmosignalling-mediated stomatal closure to create a humid habitat that favours bacterial growth and opens up a new avenue for accurately elucidating functions of effectors from numerous gram-negative plant bacteria in native infection contexts.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
eINTACT
dc.subject
Arabidopsis thaliana
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
The eINTACT system dissects bacterial exploitation of plant osmosignalling to enhance virulence
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-07T20:48:25Z
dc.journal.volume
9
dc.journal.number
1
dc.journal.pagination
128-141
dc.journal.pais
Reino Unido
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dc.description.fil
Fil: You, Yuan. Eberhard Karls Universität Tübingen; Alemania
dc.description.fil
Fil: Koczyk, Grzegorz. Polish Academy of Sciences; Argentina
dc.description.fil
Fil: Nuc, Maria. Polish Academy of Sciences; Argentina
dc.description.fil
Fil: Morbitzer, Robert. Eberhard Karls Universität Tübingen; Alemania
dc.description.fil
Fil: Holmes, Danalyn R.. Eberhard Karls Universität Tübingen; Alemania
dc.description.fil
Fil: von Roepenack Lahaye, Edda. Eberhard Karls Universität Tübingen; Alemania
dc.description.fil
Fil: Hou, Shiji. Huazhong Agricultural University; China
dc.description.fil
Fil: Giudicatti, Axel Joel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Gris, Carine. Université de Toulouse; Francia
dc.description.fil
Fil: Manavella, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Noël, Laurent D.. Université de Toulouse; Francia
dc.description.fil
Fil: Krajewski, Paweł. Polish Academy of Sciences; Argentina
dc.description.fil
Fil: Lahaye, Thomas. Eberhard Karls Universität Tübingen; Alemania
dc.journal.title
Nature Plants
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.nature.com/articles/s41477-022-01302-y
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1038/s41477-022-01302-y
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