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dc.contributor.author
Marchesini, Maria Ines
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dc.contributor.author
Poetsch, Ansgar
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dc.contributor.author
Guidolin, Leticia Soledad
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dc.contributor.author
Comerci, Diego José
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dc.date.available
2023-10-02T12:48:51Z
dc.date.issued
2022-06
dc.identifier.citation
Marchesini, Maria Ines; Poetsch, Ansgar; Guidolin, Leticia Soledad; Comerci, Diego José; Brucella abortus Encodes an Active Rhomboid Protease: Proteome Response after Rhomboid Gene Deletion; MDPI; Microorganisms; 10; 1; 6-2022; 1-20
dc.identifier.issn
2076-2607
dc.identifier.uri
http://hdl.handle.net/11336/213726
dc.description.abstract
Rhomboids are intramembrane serine proteases highly conserved in the three domains of life. Their key roles in eukaryotes are well understood but their contribution to bacterial physiology is still poorly characterized. Here we demonstrate that Brucella abortus, the etiological agent of the zoonosis called brucellosis, encodes an active rhomboid protease capable of cleaving model heterologous substrates like Drosophila melanogaster Gurken and Providencia stuartii TatA. To address the impact of rhomboid deletion on B. abortus physiology, the proteomes of mutant and parental strains were compared by shotgun proteomics. About 50% of the B. abortus predicted proteome was identified by quantitative proteomics under two experimental conditions and 108 differentially represented proteins were detected. Membrane associated proteins that showed variations in concentration in the mutant were considered as potential rhomboid targets. This class included nitric oxide reductase sub-unit C NorC (Q2YJT6) and periplasmic protein LptC involved in LPS transport to the outer membrane (Q2YP16). Differences in secretory proteins were also addressed. Differentially represented proteins included a putative lytic murein transglycosylase (Q2YIT4), nitrous-oxide reductase NosZ (Q2YJW2) and high oxygen affinity Cbb3-type cytochrome c oxidase subunit (Q2YM85). Deletion of rhomboid had no obvious effect in B. abortus virulence. However, rhomboid overexpression had a negative impact on growth under static conditions, suggesting an effect on denitrification enzymes and/or high oxygen affinity cytochrome c oxidase required for growth in low oxygen tension conditions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
MDPI
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
BRUCELLA ABORTUS
dc.subject
LABEL-FREE PROTEOMICS
dc.subject
PROTEASE
dc.subject
RHOMBOID
dc.subject.classification
Biología Celular, Microbiología
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Brucella abortus Encodes an Active Rhomboid Protease: Proteome Response after Rhomboid Gene Deletion
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-08T00:39:47Z
dc.journal.volume
10
dc.journal.number
1
dc.journal.pagination
1-20
dc.journal.pais
Suiza
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dc.journal.ciudad
Basel
dc.description.fil
Fil: Marchesini, Maria Ines. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
dc.description.fil
Fil: Poetsch, Ansgar. Ruhr-universität Bochum. Fakultät Für Biologie & Biotechnologie. Lehrstuhl Für Biochemie Der Pflanzen; Alemania
dc.description.fil
Fil: Guidolin, Leticia Soledad. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
dc.description.fil
Fil: Comerci, Diego José. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Biotecnológicas; Argentina
dc.journal.title
Microorganisms
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/microorganisms10010114
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