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dc.contributor.author
de Melo, Thatiana Corrêa  
dc.contributor.author
Trevisan Silva, Dilza  
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Alvarez Flores, Miryam P.  
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Gomes, Renata Nascimento  
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de Souza, Marcelo Medina  
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Valerio, Hellen Paula  
dc.contributor.author
Oliveira, Douglas S.  
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DeOcesano Pereira, Carlos  
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Botosso, Viviane Fongaro  
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Calil Jorge, Soraia Attie  
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Schattner, Mirta Ana  
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Gomez, Ricardo Martin  
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Chudzinski Tavassi, Ana Marisa  
dc.date.available
2023-09-27T15:54:49Z  
dc.date.issued
2022-09  
dc.identifier.citation
de Melo, Thatiana Corrêa; Trevisan Silva, Dilza; Alvarez Flores, Miryam P.; Gomes, Renata Nascimento; de Souza, Marcelo Medina; et al.; Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells; Multidisciplinary Digital Publishing Institute; International Journal of Molecular Sciences; 23; 18; 9-2022; 1-17  
dc.identifier.issn
1422-0067  
dc.identifier.uri
http://hdl.handle.net/11336/213270  
dc.description.abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for the severe pandemic of acute respiratory disease, coronavirus disease 2019 (COVID-19), experienced in the 21st century. The clinical manifestations range from mild symptoms to abnormal blood coagulation and severe respiratory failure. In severe cases, COVID-19 manifests as a thromboinflammatory disease. Damage to the vascular compartment caused by SARS-CoV-2 has been linked to thrombosis, triggered by an enhanced immune response. The molecular mechanisms underlying endothelial activation have not been fully elucidated. We aimed to identify the proteins correlated to the molecular response of human umbilical vein endothelial cells (HUVECs) after exposure to SARS-CoV-2, which might help to unravel the molecular mechanisms of endothelium activation in COVID-19. In this direction, we exposed HUVECs to SARS-CoV-2 and analyzed the expression of specific cellular receptors, and changes in the proteome of HUVECs at different time points. We identified that HUVECs exhibit non-productive infection without cytopathic effects, in addition to the lack of expression of specific cell receptors known to be essential for SARS-CoV-2 entry into cells. We highlighted the enrichment of the protein SUMOylation pathway and the increase in SUMO2, which was confirmed by orthogonal assays. In conclusion, proteomic analysis revealed that the exposure to SARS-CoV-2 induced oxidative stress and changes in protein abundance and pathways enrichment that resembled endothelial dysfunction.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Multidisciplinary Digital Publishing Institute  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/  
dc.subject
ENDOTHELIAL CELLS  
dc.subject
HUVECS  
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MASS SPECTROMETRY  
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PROTEOMICS  
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SARS-COV-2  
dc.subject.classification
Patología  
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Medicina Básica  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Proteomic Analysis Identifies Molecular Players and Biological Processes Specific to SARS-CoV-2 Exposure in Endothelial Cells  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2023-07-04T10:59:22Z  
dc.journal.volume
23  
dc.journal.number
18  
dc.journal.pagination
1-17  
dc.journal.pais
Suiza  
dc.description.fil
Fil: de Melo, Thatiana Corrêa. Butantan Institute; Brasil  
dc.description.fil
Fil: Trevisan Silva, Dilza. Butantan Institute; Brasil  
dc.description.fil
Fil: Alvarez Flores, Miryam P.. Butantan Institute; Brasil  
dc.description.fil
Fil: Gomes, Renata Nascimento. Butantan Institute; Brasil  
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Fil: de Souza, Marcelo Medina. Butantan Institute; Brasil  
dc.description.fil
Fil: Valerio, Hellen Paula. Butantan Institute; Brasil  
dc.description.fil
Fil: Oliveira, Douglas S.. Butantan Institute; Brasil  
dc.description.fil
Fil: DeOcesano Pereira, Carlos. Butantan Institute; Brasil  
dc.description.fil
Fil: Botosso, Viviane Fongaro. Butantan Institute; Brasil  
dc.description.fil
Fil: Calil Jorge, Soraia Attie. Butantan Institute; Brasil  
dc.description.fil
Fil: Schattner, Mirta Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina  
dc.description.fil
Fil: Gomez, Ricardo Martin. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Biotecnología y Biología Molecular. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Biotecnología y Biología Molecular; Argentina  
dc.description.fil
Fil: Chudzinski Tavassi, Ana Marisa. Centre of Excellence in New Target Discovery; Brasil  
dc.journal.title
International Journal of Molecular Sciences  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/23/18/10452  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/ijms231810452