Artículo
Simultaneous Observation of Cells and Nuclear Tracks from the Boron Neutron Capture Reaction by UV-C Sensitization of Polycarbonate
Portu, Agustina Mariana
; Rossini, Andrés Eugenio
; Thorp, Silvia Inés; Curotto, Paula; Pozzi, Emiliano César Cayetano; Granell, Pablo Nicolás; Golmar, Federico
; Cabrini, Romulo Luis; Saint Martin, María Laura Gisela
Fecha de publicación:
04/2015
Editorial:
Cambridge University Press
Revista:
Microscopy & Microanalysis
ISSN:
1431-9276
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The distribution of boron in tissue samples coming from boron neutron capture therapy protocols can be determined through the analysis of its autoradiography image on a nuclear track detector. A more precise knowledge of boron atom location on the microscopic scale can be attained by the observation of nuclear tracks superimposed on the sample image on the detector. A method to produce an imprint of cells cultivated on a polycarbonate detector was developed, based on the photodegradation properties of UV-C radiation on this material. Optimal conditions to generate an appropriate monolayer of Mel-J cells incubated with boronophenylalanine were found. The best images of both cells and nuclear tracks were obtained for a neutron fluence of 1013 cm-2, 6 h UV-C (254 nm) exposure, and 4 min etching time with a KOH solution. The imprint morphology was analyzed by both light and scanning electron microscopy. Similar samples, exposed to UV-A (360 nm) revealed no cellular imprinting. Etch pits were present only inside the cell imprints, indicating a preferential boron uptake (about threefold the incubation concentration). Comparative studies of boron absorption in different cell lines and in vitro evaluation of the effect of diverse boron compounds are feasible with this methodology.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Portu, Agustina Mariana; Rossini, Andrés Eugenio; Thorp, Silvia Inés; Curotto, Paula; Pozzi, Emiliano César Cayetano; et al.; Simultaneous Observation of Cells and Nuclear Tracks from the Boron Neutron Capture Reaction by UV-C Sensitization of Polycarbonate; Cambridge University Press; Microscopy & Microanalysis; 21; 4; 4-2015; 796-804
Compartir
Altmétricas