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Artículo

Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum

Regueira, EleonoraIcon ; Sassone, Alina GriselIcon ; Scaia, María FlorenciaIcon ; Volonteri, María ClaraIcon ; Ceballos, Nora RaquelIcon
Fecha de publicación: 11/2012
Editorial: Wiley
Revista: Journal of Experimental Zoology Part A: Ecological Genetics and Physiology
ISSN: 1932-5223
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Biología Reproductiva

Resumen

Several studies indicate that wild free‐living vertebrates seasonally regulate plasma glucocorticoids. However, not only glucocorticoids but also the amount of receptors is important in determining biological responses. In this context, seasonal regulation of glucocorticoid receptor (GR) is crucial to modulate the response to glucocorticoids. Rhinella arenarum is an anuran exhibiting seasonal variations in plasma glucocorticoids and also in the number of binding sites (Bmax) of the testicular cytosolic GR. In this work, we evaluated if the annual pattern of GR protein in the testis varies seasonally and, by an in vitro approach, the role of glucocorticoids, androgens, and melatonin in the regulation of the GR Bmax and protein level. For this purpose, testes were treated with two physiological concentrations of melatonin (40 and 200 pg/ml), with or without luzindole (melatonin‐receptor antagonist); with testosterone, cyanoketone (inhibitor of steroidogenesis) or casodex (androgen‐receptor antagonist); or with dexamethasone or RU486 (GR antagonist). After treatments, Bmax and protein level were determined by the binding of [3 H]dexamethasone and Western blot, respectively. Results showed that GR protein decreases in the winter. The in vitro treatment with melatonin produced a biphasic effect on the Bmax with the lowest concentration decreasing this parameter by a receptor‐mediated mechanism. However, melatonin had no effect on the GR protein level. Conversely, a high concentration of dexamethasone up‐regulated the GR protein and androgens neither changed the Bmax nor the protein level. These findings suggest that seasonal changes in plasma melatonin and glucocorticoids modulate the effect of glucocorticoids in the testis of R. arenarum.
Palabras clave: Toad , Glucocorticoids , Glucocorticoid Receptor , Androgens
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/21301
DOI: http://dx.doi.org/10.1002/jez.1772
URL: http://onlinelibrary.wiley.com/doi/10.1002/jez.1772/abstract
Colecciones
Articulos(OCA CIUDAD UNIVERSITARIA)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA CIUDAD UNIVERSITARIA
Citación
Regueira, Eleonora; Sassone, Alina Grisel; Scaia, María Florencia; Volonteri, María Clara; Ceballos, Nora Raquel; Seasonal Changes and Regulation of the Glucocorticoid Receptor in the Testis of the Toad Rhinella arenarum; Wiley; Journal of Experimental Zoology Part A: Ecological Genetics and Physiology; 319; 1; 11-2012; 39-52
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