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dc.contributor.author
Canosa, Ivana Sofía
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dc.contributor.author
Silveyra, Gabriela Romina
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dc.contributor.author
Lofrano, Juliana Leticia
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dc.contributor.author
Medesani, Daniel Alberto
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dc.contributor.author
Rodriguez, Enrique Marcelo
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dc.date.available
2023-09-25T13:57:39Z
dc.date.issued
2022-11
dc.identifier.citation
Canosa, Ivana Sofía; Silveyra, Gabriela Romina; Lofrano, Juliana Leticia; Medesani, Daniel Alberto; Rodriguez, Enrique Marcelo; Avaliação in vitro da interferência do herbicida glyphosate no controle endócrino do crescimento ovariano por neuro-hormônios e prostaglandinas, no caranguejo do estuário Neohelice granulata; Brazilian Journals Publicações de Periódicos e Editora; Brazilian Journal of Animal and Environment Research; 5; 4; 11-2022; 3782-3796
dc.identifier.issn
2595-573X
dc.identifier.uri
http://hdl.handle.net/11336/212908
dc.description.abstract
Adult females of the crab Neohelice granulata sampled at from Samborombón Bay (Buenos Aires Province, Argentina), were used. Several in vitro tests were carried out during the pre-reproductive period, in order to evaluate the growth of the isolated ovary in the presence of the herbicide (in its commercial formulation Roundup) and/or extracts of neuroendocrine organs or inhibitors of prostaglandin synthesis. For each assay, 12 females were used, assigning a different treatment to each portion of the ovary of the same female (randomized block design). The ovary pieces were incubated for 24 h in M199 culture medium, at 24 ºC and controlled atmosphere. At the end of each assay, the ovaries were processed either for histological analysis or to determine the levels of vitellogenin (Vg) using the ELISA technique. The results of the assays conducted with extracts of eyestalk or thoracic ganglion indicated that Roundup (RUP) addition did not significantly (p>0.05) modify the effect of such extracts on ovarian growth. However, in order to assess whether the effect of RUP on isolated ovaries is related to the hormonal signaling exerted by prostaglandins (PG), an in vitro assay with ibuprofen (IBF, inhibitor of PG synthesis) was performed. The level of Vg in the treatment with RUP 0.2 mg/L + IBF 10 mg/L was significantly lower (p<0.05) than that of the Control, RUP 0.2 mg/L, or IBF 10 mg groups. Although RUP per se produced a decrease (p<0.05) in Vg levels, compared to control, its combination with IBF caused a higher (p<0.05) decrease. Given the additivity of inhibitory effects observed between ibuprofen and the RUP, it is plausible to propose that the mode of action of the RUP could be due to the inhibition of the endogenous synthesis of PGs in the ovary, although the possible interference of the RUP with the PGs receptor, or the inhibition of some step in the prostaglandin hormone transduction pathway, cannot yet be ruled out.
dc.description.abstract
Adult females of the crab Neohelice granulata sampled at from Samborombón Bay (Buenos Aires Province, Argentina), were used. Several in vitro tests were carried out during the pre-reproductive period, in order to evaluate the growth of the isolated ovary in the presence of the herbicide (in its commercial formulation Roundup) and/or extracts of neuroendocrine organs or inhibitors of prostaglandin synthesis. For each assay, 12 females were used, assigning a different treatment to each portion of the ovary of the same female (randomized block design). The ovary pieces were incubated for 24 h in M199 culture medium, at 24 ºC and controlled atmosphere. At the end of each assay, the ovaries were processed either for histological analysis or to determine the levels of vitellogenin (Vg) using the ELISA technique. The results of the assays conducted with extracts of eyestalk or thoracic ganglion indicated that Roundup (RUP) addition did not significantly (p>0.05) modify the effect of such extracts on ovarian growth. However, in order to assess whether the effect of RUP on isolated ovaries is related to the hormonal signaling exerted by prostaglandins (PG), an in vitro assay with ibuprofen (IBF, inhibitor of PG synthesis) was performed. The level of Vg in the treatment with RUP 0.2 mg/L + IBF 10 mg/L was significantly lower (p<0.05) than that of the Control, RUP 0.2 mg/L, or IBF 10 mg groups. Although RUP per se produced a decrease (p<0.05) in Vg levels, compared to control, its combination with IBF caused a higher (p<0.05) decrease. Given the additivity of inhibitory effects observed between ibuprofen and the RUP, it is plausible to propose that the mode of action of the RUP could be due to the inhibition of the endogenous synthesis of PGs in the ovary, although the possible interference of the RUP with the PGs receptor, or the inhibition of some step in the prostaglandin hormone transduction pathway, cannot yet be ruled out.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Brazilian Journals Publicações de Periódicos e Editora
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
GLYPHOSATE
dc.subject
CRUSTACEANS
dc.subject
OVARY
dc.subject
HORMONES
dc.subject
PROSTAGLANDINS
dc.subject.classification
Biología Reproductiva
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Avaliação in vitro da interferência do herbicida glyphosate no controle endócrino do crescimento ovariano por neuro-hormônios e prostaglandinas, no caranguejo do estuário Neohelice granulata
dc.title
In vitro evaluation of the interference of the the herbicide glyphosate on the endocrine control of ovarian growth, by neurohomones and prostaglandins, in the estuarine crab Neohelice granulata
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-06T22:32:25Z
dc.journal.volume
5
dc.journal.number
4
dc.journal.pagination
3782-3796
dc.journal.pais
Brasil
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dc.journal.ciudad
Curitiba
dc.description.fil
Fil: Canosa, Ivana Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
dc.description.fil
Fil: Silveyra, Gabriela Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
dc.description.fil
Fil: Lofrano, Juliana Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
dc.description.fil
Fil: Medesani, Daniel Alberto. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Fisiologia de Crustáceos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina
dc.description.fil
Fil: Rodriguez, Enrique Marcelo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Biodiversidad y Biología Experimental y Aplicada. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Biodiversidad y Biología Experimental y Aplicada; Argentina. Universidad de Buenos Aires. Facultad de Cs.exactas y Naturales. Departamento de Biodiversidad y Biologia Experimental. Laboratorio de Fisiologia de Crustáceos; Argentina
dc.journal.title
Brazilian Journal of Animal and Environment Research
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.34188/bjaerv5n4-027
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