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dc.contributor.author
Rudtanatip, Tawut
dc.contributor.author
Somintara, Somsuda
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Sakaew, Waraporn
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El Abid, Jamal
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Cano, María Emilia
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Jongsomchai, Kamonwan
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Wongprasert, Kanokpan
dc.contributor.author
Kovensky, José
dc.date.available
2023-09-25T11:52:11Z
dc.date.issued
2022-09
dc.identifier.citation
Rudtanatip, Tawut; Somintara, Somsuda; Sakaew, Waraporn; El Abid, Jamal; Cano, María Emilia; et al.; Sulfated Galactans from Gracilaria fisheri with Supplementation of Octanoyl Promote Wound Healing Activity In Vitro and In Vivo; Wiley VCH Verlag; Macromolecular Bioscience; 22; 12; 9-2022; 1-17
dc.identifier.issn
1616-5187
dc.identifier.uri
http://hdl.handle.net/11336/212866
dc.description.abstract
Sulfated galactans (SG) isolated from Gracilaria fisheri is partially degraded (DSG), and subsequentially supplemented with octanoyl (DSGO) and sulfate (DSGS) groups. The molecular weights of DSG, DSGO, and DSGS are 7.87, 152.79, and 97.07 kDa, respectively. The modification is confirmed using FTIR and NMR, while in vitro wound healing activity is assessed using scratched wound fibroblasts. The results reveal that DSGO exhibits highest percentage of wound closure in scratched fibroblast L929 cells. Furthermore, DSGO is able to promote proliferation and accelerate migration of scratched fibroblasts, which correspond to the regulation of proteins and mRNA (Ki67, p-FAK, vimentin, and E-cadherin) determined by Western blotting and qPCR analysis. The superior wound healing activity of DSGO is also confirmed in excision wound of rats. The results demonstrate that DSGO significantly enhances the percentage of wound closure, re-epithelialization, and collagen arrangement, increases α-smoth muscle actin (α-SMA) and vimentin expression, and decreases that of tumor necrosis factor-α (TNF-α) at the wound site. The results suggest that degraded SG supplemented with medium-chain fatty acids of octanoyl group may pass through the membrane, subsequently activating the mediators associated with proliferation and migration of fibroblasts, which can potentially lead to the promotion of wound healing activity.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley VCH Verlag
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
EXCISION RAT
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FIBROBLAST CULTURE
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HEALING MECHANISM
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OCTANOYL-SULFATED GALACTANS
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WOUND HEALING
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Química Orgánica
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Ciencias Químicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Sulfated Galactans from Gracilaria fisheri with Supplementation of Octanoyl Promote Wound Healing Activity In Vitro and In Vivo
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-06T17:31:11Z
dc.journal.volume
22
dc.journal.number
12
dc.journal.pagination
1-17
dc.journal.pais
Alemania
dc.journal.ciudad
Weinheim
dc.description.fil
Fil: Rudtanatip, Tawut. Khon Kaen University; Tailandia
dc.description.fil
Fil: Somintara, Somsuda. Khon Kaen University; Tailandia
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Fil: Sakaew, Waraporn. Khon Kaen University; Tailandia
dc.description.fil
Fil: El Abid, Jamal. Centre National de la Recherche Scientifique; Francia
dc.description.fil
Fil: Cano, María Emilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Centro de Investigaciones en Hidratos de Carbono. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Centro de Investigaciones en Hidratos de Carbono; Argentina
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Fil: Jongsomchai, Kamonwan. University Of Phayao; Tailandia
dc.description.fil
Fil: Wongprasert, Kanokpan. Mahidol University; Tailandia
dc.description.fil
Fil: Kovensky, José. Centre National de la Recherche Scientifique; Francia
dc.journal.title
Macromolecular Bioscience
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/mabi.202200172
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