Artículo
Cholesterol-24-hydroxylase (CYP46) in the old brain: Analysis of positive populations and factors triggering its expression in astrocytes
Cataldi, Gabriel Enrique
; Elorza, Setiembre Delfina; Toledano Zaragoza, Ana; de Olmos, Soledad
; Cragnolini, Andrea Beatriz
; Martín, Mauricio Gerardo
Fecha de publicación:
01/12/2022
Editorial:
Wiley-liss, div John Wiley & Sons Inc.
Revista:
Journal Of Comparative Neurology
ISSN:
0021-9967
e-ISSN:
1096-9861
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Cholesterol-24-hydroxylase (CYP46), a member of the cytochrome P450 superfamily of enzymes, is selectively expressed in the brain and is mainly responsible for cholesterol turnover in the central nervous system. Although increased cyp46A1 gene expression has been linked to cognitive alterations in aging and observed in neurodegenerative diseases and after traumatic brain injury, a detailed characterization of the brain regions and cell types in which CYP46 is expressed in old individuals has not been performed. Using immunohistochemistry and immunofluorescence, we investigated the specific regions and cell populations in the brain, in which cyp46A1 is expressed in 24-month-old mice. We found that CYP46 is localized in the same neuronal populations in young and old brains, mainly in the hippocampus, in cortical layers, and in Purkinje neurons of the cerebellum. No increase in CYP46 levels was found in astrocytes in old mice brains, in primary astrocyte-neuron cocultures aged in vitro, or in primary cultures of senescent astrocytes. However, interleukin-6 treatment strongly induced cyp46A1 expression in reactive astrocytes characterized by high GFAP levels but had no effect in nonactivated astrocytes. Our data suggest that cholesterol-24-hydroxylase expression is triggered in reactive astrocytes in response to proinflammatory signals, probably as part of a response mechanism to injury.
Palabras clave:
AGING
,
ASTROCYTES
,
BRAIN
,
CYP46
Archivos asociados
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Identificadores
Colecciones
Articulos(IIBYT)
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS
Articulos de INSTITUTO DE INVESTIGACIONES BIOLOGICAS Y TECNOLOGICAS
Articulos(INIMEC - CONICET)
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Articulos de INSTITUTO DE INV. MEDICAS MERCEDES Y MARTIN FERREYRA
Citación
Cataldi, Gabriel Enrique; Elorza, Setiembre Delfina; Toledano Zaragoza, Ana; de Olmos, Soledad; Cragnolini, Andrea Beatriz; et al.; Cholesterol-24-hydroxylase (CYP46) in the old brain: Analysis of positive populations and factors triggering its expression in astrocytes; Wiley-liss, div John Wiley & Sons Inc.; Journal Of Comparative Neurology; 531; 3; 1-12-2022; 486-499
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