Artículo
Tig1 regulates proximo-distal identity during salamander limb regeneration
Oliveira, Catarina R.; Knapp, Dunja; Elewa, Ahmed; Gerber, Tobias; Gonzalez Malagon, Sandra G.; Gates, Phillip B.; Walters, Hannah E.; Petzold, Andreas; Arce, Hernán
; Cordoba, Rodrigo C.; Subramanian, Elaiyaraja; Chara, Osvaldo
; Tanaka, Elly M.; Simon, András; Yun, Maximina H.
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
12/2022
Editorial:
Nature Research
Revista:
Nature Communications
ISSN:
2041-1723
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Salamander limb regeneration is an accurate process which gives rise exclusively to the missing structures, irrespective of the amputation level. This suggests that cells in the stump have an awareness of their spatial location, a property termed positional identity. Little is known about how positional identity is encoded, in salamanders or other biological systems. Through single-cell RNAseq analysis, we identified Tig1/Rarres1 as a potential determinant of proximal identity. Tig1 encodes a conserved cell surface molecule, is regulated by retinoic acid and exhibits a graded expression along the proximo-distal axis of the limb. Its overexpression leads to regeneration defects in the distal elements and elicits proximal displacement of blastema cells, while its neutralisation blocks proximo-distal cell surface interactions. Critically, Tig1 reprogrammes distal cells to a proximal identity, upregulating Prod1 and inhibiting Hoxa13 and distal transcriptional networks. Thus, Tig1 is a central cell surface determinant of proximal identity in the salamander limb.
Palabras clave:
AXOLOTL
,
TIG1
,
PROXIMAL IDENTITY
,
REGENERATION
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IFLYSIB)
Articulos de INST.FISICA DE LIQUIDOS Y SIST.BIOLOGICOS (I)
Articulos de INST.FISICA DE LIQUIDOS Y SIST.BIOLOGICOS (I)
Citación
Oliveira, Catarina R.; Knapp, Dunja; Elewa, Ahmed; Gerber, Tobias; Gonzalez Malagon, Sandra G.; et al.; Tig1 regulates proximo-distal identity during salamander limb regeneration; Nature Research; Nature Communications; 13; 1; 12-2022; 1-16
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