Artículo
Endophytic bacteria from Ilex paraguariensis shoot cultures: localization, characterization, and response to isothiazolone biocides
Luna, Claudia Verónica
; Acevedo, Raúl Maximiliano
; Collavino, Mónica Mariana
; Gonzalez, Ana María
; Mroginski, Luis Amado
; Sansberro, Pedro Alfonso
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
![Icon](/themes/CONICETDigital/images/conicet.png)
Fecha de publicación:
06/2013
Editorial:
Springer
Revista:
In Vitro Cellular & Developmental Biology. Plant
ISSN:
1054-5476
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Nodal segment explants of Ilex paraguariensis, collected from greenhouse-grown plants, were found tocontain endophytic bacteria. After culturing in bioreactors, 16 rRNA gene analyses and analytical profile index biochemicaltests were used to identify these bacteria asStenotrophomonas malthophilia. The presence of bacterialcells in the intercellular spaces of stem cortical parenchymawas detected in histological sections by scanning electronmicroscopy. A range of commercial isothiazolone biocideswere tested for their ability to repress the growth of Gramnegativebacteria grown in liquid media during themicropropagation phase. The addition of 0.75 ml l−1 Delcide? TG (5-chloro-2-methyl-4-isothiazolin-3-one + 2-methyl-4-isothiazolin-3-one, 1.05% and 0.45%, respectively) to the culture media resulted in 100% visibly clean cultures, with no suppression of shoot growth.
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos(IBONE)
Articulos de INST.DE BOTANICA DEL NORDESTE (I)
Articulos de INST.DE BOTANICA DEL NORDESTE (I)
Citación
Luna, Claudia Verónica; Acevedo, Raúl Maximiliano; Collavino, Mónica Mariana; Gonzalez, Ana María; Mroginski, Luis Amado; et al.; Endophytic bacteria from Ilex paraguariensis shoot cultures: localization, characterization, and response to isothiazolone biocides; Springer; In Vitro Cellular & Developmental Biology. Plant; 49; 3; 6-2013; 326-332
Compartir
Altmétricas