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dc.contributor.author
de Diego, Gabriel Alejandro
dc.contributor.author
Penas Steinhardt, Alberto
dc.contributor.author
Ferro, Juan Pablo
dc.contributor.author
Palacio, Mauro Javier
dc.contributor.author
Ossana, Natalia Alejandra
dc.contributor.author
Eissa, Bettina Lorena
dc.contributor.author
Belforte, Fiorella Sabrina
dc.date.available
2023-09-19T14:49:49Z
dc.date.issued
2022-11
dc.identifier.citation
de Diego, Gabriel Alejandro; Penas Steinhardt, Alberto; Ferro, Juan Pablo; Palacio, Mauro Javier; Ossana, Natalia Alejandra; et al.; Impact of exposure to arsenic on the bacterial microbiota associated with river biofilms in the Pampas region; Elsevier Science; Aquatic Toxicology; 252; 11-2022; 1-8
dc.identifier.issn
0166-445X
dc.identifier.uri
http://hdl.handle.net/11336/212065
dc.description.abstract
Freshwater contamination by arsenic (As) is a worldwide problem. It may be found in Pampean streams of Argentina at concentrations higher than those recommended by international organizations and stipulated by national regulations. Exposure to high As concentrations causes serious consequences to both human health and the environment. The general objective of this work was to evaluate the effect of As on the biofilm microbiota structure from Naveira stream, Luján, Province of Buenos Aires (Coordinates: 34º34′02″ S 59º03′51″ W). The biofilm collected was cultivated in glass aquaria at different As III concentrations (0, 0.2 and 20 mg / L), inside incubation chambers under controlled conditions (16 h light: 8 h dark and 24 ± 1 °C) and constant aeration for 31 d, with partial water renewal every 9 d. We amplified the hypervariable regions V3 and V4 of the bacterial 16S rRNA gene from biofilm bacterial community samples to determine the diversity and abundance of the different taxa. The taxonomic composition of each sample, the alpha diversity of each treatment and the main metabolic pathways were analyzed. Principal Component Analysis of the present phyla and a Linear Discriminant Analysis of the metabolic pathways was also performed. Significant changes were observed in relation to the taxonomic composition of the bacterial community after exposure to the metalloid. However, this effect was not observed at the low concentration used (0.2 mg / L), which is the one that corresponds to ecologically relevant levels. The significantly affected phyla were Verrucomicrobiota, Acidobacteriota, Patescibacteria, Hydrogenedentes and WPS-2. The relative abundances of the Verrucomicrobiota, WPS-2 and Patescibacteria groups were notably decreased in the treatment with high As, while the Acidobacteria group was increased in both treatments with As. The stream samples showed greater bacterial diversity than those grown in the laboratory without As. Finally, it was possible to characterize the metabolic profile of the biofilm developed under natural conditions in the leaves of the aquatic plant Elodea canadensis in the Naveira stream. In addition, results showed that biosynthesis-related pathways were more abundant at the high As concentration treatment (20 mg / L).
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ARSENIC
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BIOFILM
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GEN16S
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METAGENOMICS
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Impact of exposure to arsenic on the bacterial microbiota associated with river biofilms in the Pampas region
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-31T15:07:49Z
dc.journal.volume
252
dc.journal.pagination
1-8
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: de Diego, Gabriel Alejandro. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina
dc.description.fil
Fil: Penas Steinhardt, Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de Lujan. Departamento de Ciencias Básicas. Laboratorio de Genómica Computacional; Argentina
dc.description.fil
Fil: Ferro, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina
dc.description.fil
Fil: Palacio, Mauro Javier. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina
dc.description.fil
Fil: Ossana, Natalia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina
dc.description.fil
Fil: Eissa, Bettina Lorena. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Luján. Departamento de Ciencias Básicas. Programa de Ecofisiología Aplicada; Argentina
dc.description.fil
Fil: Belforte, Fiorella Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Universidad Nacional de Luján. Instituto de Ecología y Desarrollo Sustentable; Argentina. Universidad Nacional de Lujan. Departamento de Ciencias Básicas. Laboratorio de Genómica Computacional; Argentina
dc.journal.title
Aquatic Toxicology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0166445X22002454
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.aquatox.2022.106319
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