Artículo
In vitro Inhibitory Activity of IgY Antibodies Against Salmonella Ser: Newport Isolated from Horses
Bustos, Carla Paola
; Leiva, Carlos Leónidas
; Gambarotta, Mariana; Guida, Nora; Chacana, Pablo Anibal
Fecha de publicación:
08/2021
Editorial:
Elsevier Science Inc.
Revista:
Journal of Equine Veterinary Science
ISSN:
0737-0806
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Equine salmonellosis is caused by several Salmonella serotypes, including Salmonella Newport, which cause enterocolitis and diarrhea. Treatment usually includes the administration of antibiotics. However, since multidrug-resistant Salmonella is commonly detected, alternative options to control the pathogen are needed. One of these options is the use of specific egg yolk antibodies (IgY) for passive immunotherapy. Thus, the aim of our work was to produce IgY antibodies against an equine S. Newport strain and assess their in vitro inhibitory activity. To this end, laying hens were immunized with an inactivated S. Newport strain by using either Freund's or Montanide adjuvant and egg yolk extracts were obtained. The levels of specific IgY antibodies against Salmonella in sera and egg extracts were determined by dot-blot and microagglutination. Besides, the IgY extracts were characterized by total protein analysis, SDS-PAGE, Western Blot, and inhibition of bacterial motility. IgY extracts showed high purity (87.7 to 91.8 %), high microagglutination titers, and the ability to inhibit the motility of the bacterium. The results using Montanide were similar to those using the traditional Freund's adjuvant. Thus, Montanide may also be a good adjuvant to produce IgY. IgY-technology represents a potential tool for the control of salmonellosis in horses.
Palabras clave:
EGG YOLK
,
HORSES
,
IGY ANTIBODY
,
SALMONELLA NEWPORT
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos (IPVET)
Articulos de INSTITUTO DE PATOBIOLOGIA VETERINARIA
Articulos de INSTITUTO DE PATOBIOLOGIA VETERINARIA
Citación
Bustos, Carla Paola; Leiva, Carlos Leónidas; Gambarotta, Mariana; Guida, Nora; Chacana, Pablo Anibal; In vitro Inhibitory Activity of IgY Antibodies Against Salmonella Ser: Newport Isolated from Horses; Elsevier Science Inc.; Journal of Equine Veterinary Science; 103; 8-2021; 1-5
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