Artículo
Species identification in routine casework samples using the SPInDel kit
Pereira, Filipe; Alves, Cíntia; Couto, Cátia; López Díaz, Lourdes; Parra, David; Furfuro, Sandra; Aler, Mercedes; Burillo Borrego, Luís; Olekšáková, Tereza; Balsa, Filipa; Sampaio, Lisa; Porto, Maria João Anjos; Costa, Heloisa Afonso; Arévalo Voss, Cristina; Caputo, Mariela
; Corach, Daniel
; García, Óscar; Pedrosa Moro, Susana; Pereira, Rui; Amorim, António
Fecha de publicación:
09/2019
Editorial:
Elsevier
Revista:
Forensic Science International: Genetics Supplement Series
ISSN:
1875-1768
e-ISSN:
1875-175X
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The identification of species in casework samples is of fundamental importance for forensic investigations. Laboratories are increasingly compelled to provide accurate and fast identifications in trace materials left on crime scenes, wildlife poaching, illegal trade of protected species, fraudulent food products cases, etc. However, the field of nonhuman forensic genetics is still working on the standardization of typing methods and practices. Here we describe the successful implementation of the Species Identification by Insertions/Deletions (SPInDel) method in routine casework analyses in 11 laboratories worldwide. The SPInDel was developed to detect human DNA, at the same time that identifies common animal species. The fragment size analysis of six mtDNA regions allows identification in suboptimal DNA samples, including mixtures, with no need for sequencing. The samples were collected from 2013 to 2018 and included hair, blood, meat, saliva, faeces, bones, etc. The SPInDel kit successfully identified >95% of the samples, being dog, human and pig the most frequently detected species. The six SPInDel loci were successfully amplified in mixtures and degraded samples (river water, sand, stains in clothes, etc.). Interestingly, several species that were not originally targeted by SPInDel primers were also identified (e.g., red fox, brown bear, fallow deer and red deer). In conclusion, the SPInDel kit was successfully used in crime scene investigations (often involving human DNA detection) and in cases of poaching, environmental contamination and food fraud. It is now becoming a useful tool for the routine analysis of nonhuman DNA samples within the high quality standards of forensic genetics.
Palabras clave:
FORENSICS
,
MTDNA
,
NON-HUMAN DNA
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Articulos(OCA HOUSSAY)
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Articulos de OFICINA DE COORDINACION ADMINISTRATIVA HOUSSAY
Citación
Pereira, Filipe; Alves, Cíntia; Couto, Cátia; López Díaz, Lourdes ; Parra, David; et al.; Species identification in routine casework samples using the SPInDel kit; Elsevier; Forensic Science International: Genetics Supplement Series; 7; 1; 9-2019; 180-181
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