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dc.contributor.author
Breccia, Gabriela

dc.contributor.author
Vega, Tatiana Alejandra

dc.contributor.author
Felitti, Silvina Andrea

dc.contributor.author
Picardi, Liliana Amelia

dc.contributor.author
Nestares, Graciela María

dc.date.available
2017-07-21T20:23:13Z
dc.date.issued
2013-03
dc.identifier.citation
Breccia, Gabriela; Vega, Tatiana Alejandra; Felitti, Silvina Andrea; Picardi, Liliana Amelia; Nestares, Graciela María; Differential expression of acetohydroxyacid synthase genes in sunflower plantlets and its response to imazapyr herbicide; Elsevier Ireland; Plant Science; 208; 3-2013; 28-33
dc.identifier.issn
0168-9452
dc.identifier.uri
http://hdl.handle.net/11336/21133
dc.description.abstract
Acetohydroxyacid synthase (AHAS) catalyzes the first reaction in branch chain amino acids biosynthesis. This enzyme is the target of several herbicides, including all members of the imidazolinone family. Little is known about the expression of the three acetohydroxyacid synthase genes (ahas1, ahas2 and ahas3) in sunflower. The aim of this work was to evaluate ahas gene expression and AHAS activity in different tissues of sunflower plantlets. Three genotypes differing in imidazolinone resistance were evaluated, two of which carry an herbicide resistant-endowing mutation known as Ahasl1-1 allele. In vivo and in vitro AHAS activity and transcript levels were higher in leaves than in roots. The ahas3 transcript was the less abundant in both tissues. No significant difference was observed between ahas1 and ahas2 transcript levels of the susceptible genotype but a higher ahas1 transcript level was observed in leaves of genotypes carrying Ahasl1-1 allele. Similar transcript levels were found for ahas1 and ahas2 in roots of genotypes carrying Ahasl1-1 allele whereas higher ahas2 abundance was found in the susceptible genotype. Herbicide treatment triggered tissue-specific, gene and genotype-dependent changes in ahas gene expression. AHAS activity was highly inhibited in the susceptible genotype. Differential responses were observed between in vitro and in vivo AHAS inhibition assays. These findings enhance our understanding of AHAS expression in sunflower genotypes differing for herbicide resistance and its response to herbicide treatment.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Ireland

dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
Helianthus Annuus L.
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Imidazolinone Herbicide
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Ahas Gene Expression
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Ahas Activity
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Herbicide Tolerance
dc.subject.classification
Agronomía, reproducción y protección de plantas

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Agricultura, Silvicultura y Pesca

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CIENCIAS AGRÍCOLAS

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Biotecnología Agrícola y Biotecnología Alimentaria

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Biotecnología Agropecuaria

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CIENCIAS AGRÍCOLAS

dc.title
Differential expression of acetohydroxyacid synthase genes in sunflower plantlets and its response to imazapyr herbicide
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-21T19:05:22Z
dc.journal.volume
208
dc.journal.pagination
28-33
dc.journal.pais
Irlanda

dc.description.fil
Fil: Breccia, Gabriela. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Vega, Tatiana Alejandra. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Felitti, Silvina Andrea. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias. Laboratorio de Biología Molecular; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Picardi, Liliana Amelia. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina
dc.description.fil
Fil: Nestares, Graciela María. Universidad Nacional de Rosario. Facultad de Ciencias Agrarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.journal.title
Plant Science

dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.plantsci.2013.03.008
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0168945213000642
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