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dc.contributor.author
Williams, Alan
dc.contributor.author
Wilkinson, Adam
dc.contributor.author
Krehenbrink, Martin
dc.contributor.author
Russo, Daniela Marta
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dc.contributor.author
Zorreguieta, Angeles
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dc.contributor.author
Downie, J. Allan
dc.date.available
2017-07-21T19:57:01Z
dc.date.issued
2008-04
dc.identifier.citation
Williams, Alan; Wilkinson, Adam; Krehenbrink, Martin; Russo, Daniela Marta; Zorreguieta, Angeles; et al.; Glucomannan-mediated attachment of Rhizobium leguminosarum to pea root hairs is required for competitive nodule infection; American Society for Microbiology; Journal Of Bacteriology; 190; 13; 4-2008; 4706-4715
dc.identifier.issn
0021-9193
dc.identifier.uri
http://hdl.handle.net/11336/21119
dc.description.abstract
The Rhizobium leguminosarum biovar viciae genome contains several genes predicted to determine surface polysaccharides. Mutants predicted to affect the initial steps of polysaccharide synthesis were identified and characterized. In addition to the known cellulose (cel) and acidic exopolysaccharide (EPS) (pss) genes, we mutated three other loci; one of these loci (gmsA) determines glucomannan synthesis and one (gelA) determines a gel-forming polysaccharide, but the role of the other locus (an exoY-like gene) was not identified. Mutants were tested for attachment and biofilm formation in vitro and on root hairs; the mutant lacking the EPS was defective for both of these characteristics, but mutation of gelA or the exoY-like gene had no effect on either type of attachment. The cellulose (celA) mutant attached and formed normal biofilms in vitro, but it did not form a biofilm on root hairs, although attachment did occur. The cellulose-dependent biofilm on root hairs appears not to be critical for nodulation, because the celA mutant competed with the wild-type for nodule infection. The glucomannan (gmsA) mutant attached and formed normal biofilms in vitro, but it was defective for attachment and biofilm formation on root hairs. Although this mutant formed nodules on peas, it was very strongly outcompeted by the wild type in mixed inoculations, showing that glucomannan is critical for competitive nodulation. The polysaccharide synthesis genes around gmsA are highly conserved among other rhizobia and agrobacteria but are absent from closely related bacteria (such as Brucella spp.) that are not normally plant associated, suggesting that these genes may play a wide role in bacterium-plant interactions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
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dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Glucomannan
dc.subject
Adhesion
dc.subject
Polarity
dc.subject.classification
Bioquímica y Biología Molecular
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dc.subject.classification
Ciencias Biológicas
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dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
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dc.title
Glucomannan-mediated attachment of Rhizobium leguminosarum to pea root hairs is required for competitive nodule infection
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-18T14:50:37Z
dc.identifier.eissn
1098-5530
dc.journal.volume
190
dc.journal.number
13
dc.journal.pagination
4706-4715
dc.journal.pais
Estados Unidos
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dc.journal.ciudad
Washington
dc.description.fil
Fil: Williams, Alan. John Innes Institute; Reino Unido
dc.description.fil
Fil: Wilkinson, Adam. John Innes Institute; Reino Unido
dc.description.fil
Fil: Krehenbrink, Martin. John Innes Institute; Reino Unido
dc.description.fil
Fil: Russo, Daniela Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil
Fil: Zorreguieta, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
dc.description.fil
Fil: Downie, J. Allan. John Innes Institute; Reino Unido
dc.journal.title
Journal Of Bacteriology
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jb.asm.org/content/190/13/4706.long
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/JB.01694-07
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