Aproach to molecular characterization of fasciola hepatica isolates susceptible and resistant to triclabendazole by technique RAPDS-PCR

Abstract

Liver flukes Fasciola hepatica are among the causes of foodborne diseases of parasitic etiology. These parasites cause significant public health problems and substantial economic losses to the livestock industry. Its control is mainly based on the use of triclabendazole (TCBZ), a halogenated benzimidazole thiol derivative which shows excellent efficacy against both juvenile (immature) and adult stages. Anthelmintic resistance in parasitic of livestock is a chronic problem in the world. The intensive use of TCBZ has resulted in the development of resistant liver flukes. In nematodes the resistance to the benzimidazoles is caused by genetic changes in genes encoding β-tubulins. In the case of F. hepatica TCBZ resistant were not detected these genetic changes for which the resistance obtained is from other genetic or metabolic changes. The present study shows the comparative molecular characterization of F. hepatica susceptible and resistant to TCBZ using the Random Amplified Polymorphic ADN Fragments (RAPDs-PCR) technique. Were evaluated ten different primers and four isolates of F. hepatica Cullompton and CEDIVE (susceptibles) and Sligo and Cajamarca (resistant to TCBZ). The results provides evidence of genetic variations of F. hepatica between the different isolates, reinforcing previous studies where is proposed that the resistance is due to a combination of factors, which vary among different isolates. Further work on this relevant area is required.