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dc.contributor.author
Burastero, Osvaldo  
dc.contributor.author
Niebling, Stephan  
dc.contributor.author
Defelipe, Lucas Alfredo  
dc.contributor.author
Günther, Christian  
dc.contributor.author
Struve, Angelica  
dc.contributor.author
Garcia Alai, Maria M.  
dc.date.available
2023-09-07T11:51:27Z  
dc.date.issued
2021-10  
dc.identifier.citation
Burastero, Osvaldo; Niebling, Stephan; Defelipe, Lucas Alfredo; Günther, Christian; Struve, Angelica; et al.; eSPC: An online data-analysis platform for molecular biophysics; International Union of Crystallography; Acta Crystallographica Section D: Structural Biology; 77; 10-2021; 1241-1250  
dc.identifier.issn
2059-7983  
dc.identifier.uri
http://hdl.handle.net/11336/210801  
dc.description.abstract
All biological processes rely on the formation of protein–ligand, protein–peptide and protein–protein complexes. Studying the affinity, kinetics and thermodynamics of binding between these pairs is critical for understanding basic cellular mechanisms. Many different technologies have been designed for probing interactions between biomolecules, each based on measuring different signals (fluorescence, heat, thermophoresis, scattering and interference, among others). Evaluation of the data from binding experiments and their fitting is an essential step towards the quantification of binding affinities. Here, user-friendly online tools to analyze biophysical data from steady-state fluorescence spectroscopy, microscale thermophoresis and differential scanning fluorimetry experiments are presented. The modules of the data-analysis platform (https://spc.embl-hamburg.de/) contain classical thermodynamic models and clear user guidelines for the determination of equilibrium dissociation constants (Kd) and thermal unfolding parameters such as melting temperatures (Tm).  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
International Union of Crystallography  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/  
dc.subject
BINDING AFFINITY  
dc.subject
DIFFERENTIAL SCANNING FLUORIMETRY  
dc.subject
ESPC  
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KD  
dc.subject
LIGAND SCREENING  
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MICROSCALE THERMOPHORESIS  
dc.subject
MOLECULAR BIOPHYSICS  
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MOLECULAR INTERACTIONS  
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ONLINE SERVERS  
dc.subject
OPEN SCIENCE  
dc.subject
PROTEIN STABILITY  
dc.subject
TM  
dc.subject.classification
Biofísica  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
eSPC: An online data-analysis platform for molecular biophysics  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2023-08-30T10:39:08Z  
dc.journal.volume
77  
dc.journal.pagination
1241-1250  
dc.journal.pais
Reino Unido  
dc.description.fil
Fil: Burastero, Osvaldo. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Química Biológica de la Facultad de Ciencias Exactas y Naturales; Argentina  
dc.description.fil
Fil: Niebling, Stephan. Centre For Structural Systems Biology; Alemania. European Molecular Biology Laboratory Hamburg; Alemania  
dc.description.fil
Fil: Defelipe, Lucas Alfredo. Centre For Structural Systems Biology; Alemania. European Molecular Biology Laboratory Hamburg; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina  
dc.description.fil
Fil: Günther, Christian. Centre For Structural Systems Biology; Alemania. European Molecular Biology Laboratory Hamburg; Alemania  
dc.description.fil
Fil: Struve, Angelica. Centre For Structural Systems Biology; Alemania. European Molecular Biology Laboratory Hamburg; Alemania  
dc.description.fil
Fil: Garcia Alai, Maria M.. Centre For Structural Systems Biology; Alemania. European Molecular Biology Laboratory Hamburg; Alemania  
dc.journal.title
Acta Crystallographica Section D: Structural Biology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://scripts.iucr.org/cgi-bin/paper?S2059798321008998  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1107/S2059798321008998