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Artículo

Role of G-proteins in the effects of leptin on pedunculopontine nucleus neurons

Beck, Paige; Mahaffey, Susan; Urbano Suarez, Francisco JoseIcon ; Garcia Rill, Edgar
Fecha de publicación: 06/2013
Editorial: Wiley
Revista: Journal of Neurochemistry
ISSN: 0022-3042
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Neurociencias

Resumen

The pedunculopontine nucleus (PPN), the cholinergic arm of the reticular activating system, regulates waking and rapid eye movement sleep. Here, we demonstrate immunohistochemical labeling of the leptin receptor signaling isoform in PPN neurons, and investigated the effects of G-protein modulation and the leptin triple antagonist (TA) on the action of leptin in the PPN. Whole-cell patch clamp recordings were performed in rat brainstem slices from 9 to 17 day old pups. Previous results showed that leptin caused a partial blockade of sodium (INa) and h-current (IH) in PPN neurons. TA (100 nM) reduced the blockade of INa (~ 50% reduction) and IH (~ 93% reduction) caused by leptin. Intracellular guanosine 5′-[b-thio] diphosphate trilithium salt (a G-protein inhibitor) significantly reduced the effect of leptin on INa(~ 60% reduction) but not on IH (~ 25% reduction). Intracellular GTPcS (a G-protein activator) reduced the effect of leptin on both INa (~ 80% reduction) and IH (~ 90% reduction). These results suggest that the effects of leptin on the intrinsic properties of PPN neurons are leptin receptor- and G-protein dependent. We also found that leptin enhanced NMDA receptor-mediated responses in single neurons and in the PPN population as a whole, an effect blocked by TA. These experiments further strengthen the association between leptin dysregulation and sleep disturbances.
Palabras clave: Pedundulopontine Nucleus , Brainstem , Leptin , Sodium Channels , H-Current , G-Protein
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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/21077
DOI: http://dx.doi.org/10.1111/jnc.12312
URL: http://onlinelibrary.wiley.com/doi/10.1111/jnc.12312/abstract
URL: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3766503/
Colecciones
Articulos(IFIBYNE)
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Citación
Beck, Paige; Mahaffey, Susan; Urbano Suarez, Francisco Jose; Garcia Rill, Edgar; Role of G-proteins in the effects of leptin on pedunculopontine nucleus neurons; Wiley; Journal of Neurochemistry; 126; 6; 6-2013; 705-714
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