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dc.contributor.author
Camiletti, Boris Xavier
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dc.contributor.author
Lichtemberg, Paulo dos Santos Faria
dc.contributor.author
Luo, Yong
dc.contributor.author
Paredes, Juan Andres
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dc.contributor.author
Marzall Pereira, Miriam
dc.contributor.author
Gabri Martin, Victor Manuel
dc.contributor.author
Michailides, Themis
dc.date.available
2023-09-06T16:45:50Z
dc.date.issued
2023-05-01
dc.identifier.citation
Camiletti, Boris Xavier; Lichtemberg, Paulo dos Santos Faria; Luo, Yong; Paredes, Juan Andres; Marzall Pereira, Miriam; et al.; Frequency of Alternaria genotypes resistant to SDHI fungicides in California pistachio orchards determined by real-time PCR; American Phytopathological Society; Plant Disease; 107; 5; 01-5-2023; 1433-1441
dc.identifier.issn
0191-2917
dc.identifier.uri
http://hdl.handle.net/11336/210749
dc.description.abstract
Real-time PCR methods were developed to quantify the frequency of SDHC-H134R and SDHB-H277Y mutants associated with succinate dehydrogenase inhibitors (SDHI) resistance in Alternaria populations from pistachio. The linearity of the standard curves demonstrated the applicability in the quantification of the assays. The accuracy and reliability of the qPCR protocols to determine the frequency of mutants in real samples were corroborated. Orchards visibly affected by Alternaria late blight were sampled. The frequency of mutants was determined using the qPCR assays, while the frequency of resistant phenotypes was determined using a single discriminatory dose. The statistical analysis showed that the frequencies of the mutation SDHC-H134R determined with the qPCR assay were highly correlated with those estimated with the conventional method. The survey also evidenced that resistance to boscalid is still widespread in California. Results also indicated the possible contribution of other mutations to SDHI resistance. Our results confirmed the prevalence of SDHC-H134R mutants and the occurrence of mutation SDHB-H277Y at low frequencies. The real-time PCR methods developed in this study were able to detect differences in the frequencies of resistant mutants caused by the use of chemical fungicides. Finally, the effects of two fungicide programs on the frequency of mutants resistant to SDHI and quinone outside inhibitors fungicides were studied using qPCR assays. The experiments demonstrated that the use of anilinopyrimidine and demethylation inhibitors fungicides in the same program reduced the frequency of these mutations in Alternaria populations. The qPCR methods developed and used in this study can be used to track resistance levels in the pistachio orchards on a large scale.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Phytopathological Society
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ALTERNARIA ALTERNATA
dc.subject
CARBOXAMIDES
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FUNGICIDE RESISTANCE
dc.subject
MUTANTS
dc.subject
QPCR
dc.subject.classification
Agricultura
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dc.subject.classification
Agricultura, Silvicultura y Pesca
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dc.subject.classification
CIENCIAS AGRÍCOLAS
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dc.title
Frequency of Alternaria genotypes resistant to SDHI fungicides in California pistachio orchards determined by real-time PCR
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-07T18:25:26Z
dc.identifier.eissn
1943-7692
dc.journal.volume
107
dc.journal.number
5
dc.journal.pagination
1433-1441
dc.journal.pais
Estados Unidos
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dc.description.fil
Fil: Camiletti, Boris Xavier. University Of California. Kearney Agricultural Research And Extension Center; Estados Unidos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
dc.description.fil
Fil: Lichtemberg, Paulo dos Santos Faria. University Of California. Kearney Agricultural Research And Extension Center; Estados Unidos
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Fil: Luo, Yong. University Of California. Kearney Agricultural Research And Extension Center; Estados Unidos
dc.description.fil
Fil: Paredes, Juan Andres. University Of California. Kearney Agricultural Research And Extension Center; Estados Unidos. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Marzall Pereira, Miriam. University Of California. Kearney Agricultural Research And Extension Center; Estados Unidos
dc.description.fil
Fil: Gabri Martin, Victor Manuel. University Of California. Kearney Agricultural Research And Extension Center; Estados Unidos
dc.description.fil
Fil: Michailides, Themis. University Of California. Kearney Agricultural Research And Extension Center; Estados Unidos
dc.journal.title
Plant Disease
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://apsjournals.apsnet.org/doi/10.1094/PDIS-08-22-2013-RE
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1094/PDIS-08-22-2013-RE
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