Mostrar el registro sencillo del ítem
dc.contributor.author
Trujillo-Vargas, Claudia M.
dc.contributor.author
Mauk, Kelsey E.
dc.contributor.author
Hernandez, Humberto
dc.contributor.author
de Souza, Rodrigo G.
dc.contributor.author
Yu, Zhiyuan
dc.contributor.author
Galletti, Jeremías Gastón
dc.contributor.author
Dietrich, Jana
dc.contributor.author
Paulsen, Friedrich
dc.contributor.author
de Paiva, Cintia S.
dc.date.available
2023-09-06T12:33:41Z
dc.date.issued
2022-03
dc.identifier.citation
Trujillo-Vargas, Claudia M.; Mauk, Kelsey E.; Hernandez, Humberto; de Souza, Rodrigo G.; Yu, Zhiyuan; et al.; Immune phenotype of the CD4+ T cells in the aged lymphoid organs and lacrimal glands; Springer; GeroScience; 44; 4; 3-2022; 2105-2128
dc.identifier.issn
2509-2715
dc.identifier.uri
http://hdl.handle.net/11336/210651
dc.description.abstract
Aging is associated with a massive infiltration of T lymphocytes in the lacrimal gland. Here, we aimed to characterize the immune phenotype of aged CD4+ T cells in this tissue as compared with lymphoid organs. To perform this, we sorted regulatory T cells (Tregs, CD4+CD25+GITR+) and non-Tregs (CD4+CD25negGITRneg) in lymphoid organs from female C57BL/6J mice and subjected these cells to an immunology NanoString® panel. These results were confirmed by flow cytometry, live imaging, and tissue immunostaining in the lacrimal gland. Importantly, effector T helper 1 (Th1) genes were highly upregulated on aged Tregs, including the master regulator Tbx21. Among the non-Tregs, we also found a significant increase in the levels of EOMESmed/high, TbetnegIFN-γ+, and CD62L+CD44negCD4+ T cells with aging, which are associated with cell exhaustion, immunopathology, and the generation of tertiary lymphoid tissue. At the functional level, aged Tregs from lymphoid organs are less able to decrease proliferation and IFN-γ production of T responders at any age. More importantly, human lacrimal glands (age range 55–81 years) also showed the presence of CD4+Foxp3+ cells. Further studies are needed to propose potential molecular targets to avoid immune-mediated lacrimal gland dysfunction with aging.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
AGING
dc.subject
CD4+ T CELLS
dc.subject
CONVENTIONAL T CELLS
dc.subject
DRY EYE DISEASE
dc.subject
LACRIMAL GLAND
dc.subject
LYMPHOID ORGANS
dc.subject
REGULATORY T CELLS
dc.subject
SJÖGREN’S SYNDROME
dc.subject
TRANSCRIPTOME
dc.subject.classification
Oftalmología
dc.subject.classification
Medicina Clínica
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Immune phenotype of the CD4+ T cells in the aged lymphoid organs and lacrimal glands
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-14T20:22:53Z
dc.identifier.eissn
2509-2723
dc.journal.volume
44
dc.journal.number
4
dc.journal.pagination
2105-2128
dc.journal.pais
Alemania
dc.description.fil
Fil: Trujillo-Vargas, Claudia M.. Universidad de Antioquia; Colombia
dc.description.fil
Fil: Mauk, Kelsey E.. No especifíca;
dc.description.fil
Fil: Hernandez, Humberto. Baylor College of Medicine; Estados Unidos
dc.description.fil
Fil: de Souza, Rodrigo G.. Baylor College of Medicine; Estados Unidos
dc.description.fil
Fil: Yu, Zhiyuan. No especifíca;
dc.description.fil
Fil: Galletti, Jeremías Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.description.fil
Fil: Dietrich, Jana. Universitat Erlangen-Nuremberg; Alemania
dc.description.fil
Fil: Paulsen, Friedrich. Universitat Erlangen-Nuremberg; Alemania
dc.description.fil
Fil: de Paiva, Cintia S.. No especifíca;
dc.journal.title
GeroScience
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s11357-022-00529-z
Archivos asociados