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dc.contributor.author
Fiorentino, Gabriela Alejandra  
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Miliwebsky, Elizabeth  
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Ramos, Maria Victoria  
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Zolezzi, Gisela  
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Chinen, Isabel  
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Guzmán, Glenda Mabel  
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Nocera, Rubén  
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Fernández Brando, Romina Jimena  
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Santiago, Adriana  
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Exeni, Ramón Alfonso  
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Palermo, Marina Sandra  
dc.date.available
2023-09-06T12:33:22Z  
dc.date.issued
2022-07  
dc.identifier.citation
Fiorentino, Gabriela Alejandra; Miliwebsky, Elizabeth; Ramos, Maria Victoria; Zolezzi, Gisela; Chinen, Isabel; et al.; Etiological diagnosis of post-diarrheal hemolytic uremic syndrome (HUS): humoral response contribution; Springer; Pediatric Nephrology; 38; 3; 7-2022; 739-748  
dc.identifier.issn
0931-041X  
dc.identifier.uri
http://hdl.handle.net/11336/210650  
dc.description.abstract
Background: Hemolytic uremic syndrome (HUS) is characterized by microangiopathic hemolysis, thrombocytopenia, and thrombus formation leading to tissue injury. HUS is classified according to its etiology as post-diarrheal or atypical HUS. Differential diagnosis of both entities continues to be a challenge for pediatric physicians. Methods: The aim was to improve the rapid etiological diagnosis of post-diarrheal HUS cases based on the detection of Shiga toxin (Stx)-producing Escherichia coli (STEC) infection by screening of stx1/stx2 and rfbO157 in cultured stools by multiplex PCR, and the additional detection of anti-lipopolysaccharide (anti-LPS) O157, O145, and O121 antibodies by Glyco-iELISA test. In addition, we studied patients’ relatives to detect circulating pathogenic strains that could contribute to HUS diagnosis and/or lead to the implementation of measures to prevent dissemination of familial outbreaks. This study describes the diagnosis of 31 HUS patients admitted to Hospital Municipal de Niños Prof Dr Ramón Exeni during the 2017–2020 period. Results: Stool PCR confirmed the diagnosis of STEC associated with HUS in 38.7% of patients (12/31), while anti-LPS serology did in 88.9% (24/27). In those patients in which both methods were carried out (n = 27), a strong association between the results obtained was found. We found that 30.4% of HUS patients had at least one relative positive for STEC. Conclusions: We could identify 96.3% (26/27) of HUS cases as secondary to STEC infections when both methods (genotyping and serology) were used. The results demonstrated a high circulation of STEC in HUS families and the prevalence of the STEC O157 serotype (83%) in our pediatric cohort.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Springer  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
ACUTE KIDNEY INJURY  
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ANTIBODIES  
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HUS  
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LPS  
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PCR  
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SHIGA TOXIN  
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STEC  
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Enfermedades Infecciosas  
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Ciencias de la Salud  
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CIENCIAS MÉDICAS Y DE LA SALUD  
dc.title
Etiological diagnosis of post-diarrheal hemolytic uremic syndrome (HUS): humoral response contribution  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2023-07-14T20:21:29Z  
dc.journal.volume
38  
dc.journal.number
3  
dc.journal.pagination
739-748  
dc.journal.pais
Alemania  
dc.journal.ciudad
Berlin  
dc.description.fil
Fil: Fiorentino, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina  
dc.description.fil
Fil: Miliwebsky, Elizabeth. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Virales Humanas; Argentina  
dc.description.fil
Fil: Ramos, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina  
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Fil: Zolezzi, Gisela. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Virales Humanas; Argentina  
dc.description.fil
Fil: Chinen, Isabel. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Virales Humanas; Argentina  
dc.description.fil
Fil: Guzmán, Glenda Mabel. Hospital de Niños “Prof Dr Ramón Exeni”; Argentina  
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Fil: Nocera, Rubén. Hospital de Niños “Prof Dr Ramón Exeni”; Argentina  
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Fil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina  
dc.description.fil
Fil: Santiago, Adriana. Hospital de Niños “Prof Dr Ramón Exeni”; Argentina  
dc.description.fil
Fil: Exeni, Ramón Alfonso. Hospital de Niños “Prof Dr Ramón Exeni”; Argentina  
dc.description.fil
Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina  
dc.journal.title
Pediatric Nephrology  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00467-022-05671-6