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dc.contributor.author
Fiorentino, Gabriela Alejandra
dc.contributor.author
Miliwebsky, Elizabeth
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Ramos, Maria Victoria
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Zolezzi, Gisela
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Chinen, Isabel
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Guzmán, Glenda Mabel
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Nocera, Rubén
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Fernández Brando, Romina Jimena
dc.contributor.author
Santiago, Adriana
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Exeni, Ramón Alfonso
dc.contributor.author
Palermo, Marina Sandra
dc.date.available
2023-09-06T12:33:22Z
dc.date.issued
2022-07
dc.identifier.citation
Fiorentino, Gabriela Alejandra; Miliwebsky, Elizabeth; Ramos, Maria Victoria; Zolezzi, Gisela; Chinen, Isabel; et al.; Etiological diagnosis of post-diarrheal hemolytic uremic syndrome (HUS): humoral response contribution; Springer; Pediatric Nephrology; 38; 3; 7-2022; 739-748
dc.identifier.issn
0931-041X
dc.identifier.uri
http://hdl.handle.net/11336/210650
dc.description.abstract
Background: Hemolytic uremic syndrome (HUS) is characterized by microangiopathic hemolysis, thrombocytopenia, and thrombus formation leading to tissue injury. HUS is classified according to its etiology as post-diarrheal or atypical HUS. Differential diagnosis of both entities continues to be a challenge for pediatric physicians. Methods: The aim was to improve the rapid etiological diagnosis of post-diarrheal HUS cases based on the detection of Shiga toxin (Stx)-producing Escherichia coli (STEC) infection by screening of stx1/stx2 and rfbO157 in cultured stools by multiplex PCR, and the additional detection of anti-lipopolysaccharide (anti-LPS) O157, O145, and O121 antibodies by Glyco-iELISA test. In addition, we studied patients’ relatives to detect circulating pathogenic strains that could contribute to HUS diagnosis and/or lead to the implementation of measures to prevent dissemination of familial outbreaks. This study describes the diagnosis of 31 HUS patients admitted to Hospital Municipal de Niños Prof Dr Ramón Exeni during the 2017–2020 period. Results: Stool PCR confirmed the diagnosis of STEC associated with HUS in 38.7% of patients (12/31), while anti-LPS serology did in 88.9% (24/27). In those patients in which both methods were carried out (n = 27), a strong association between the results obtained was found. We found that 30.4% of HUS patients had at least one relative positive for STEC. Conclusions: We could identify 96.3% (26/27) of HUS cases as secondary to STEC infections when both methods (genotyping and serology) were used. The results demonstrated a high circulation of STEC in HUS families and the prevalence of the STEC O157 serotype (83%) in our pediatric cohort.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Springer
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ACUTE KIDNEY INJURY
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ANTIBODIES
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HUS
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LPS
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PCR
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SHIGA TOXIN
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STEC
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Enfermedades Infecciosas
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Ciencias de la Salud
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CIENCIAS MÉDICAS Y DE LA SALUD
dc.title
Etiological diagnosis of post-diarrheal hemolytic uremic syndrome (HUS): humoral response contribution
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-14T20:21:29Z
dc.journal.volume
38
dc.journal.number
3
dc.journal.pagination
739-748
dc.journal.pais
Alemania
dc.journal.ciudad
Berlin
dc.description.fil
Fil: Fiorentino, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.description.fil
Fil: Miliwebsky, Elizabeth. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Virales Humanas; Argentina
dc.description.fil
Fil: Ramos, Maria Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.description.fil
Fil: Zolezzi, Gisela. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Virales Humanas; Argentina
dc.description.fil
Fil: Chinen, Isabel. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud "Dr. C. G. Malbran". Instituto Nacional de Enfermedades Virales Humanas; Argentina
dc.description.fil
Fil: Guzmán, Glenda Mabel. Hospital de Niños “Prof Dr Ramón Exeni”; Argentina
dc.description.fil
Fil: Nocera, Rubén. Hospital de Niños “Prof Dr Ramón Exeni”; Argentina
dc.description.fil
Fil: Fernández Brando, Romina Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.description.fil
Fil: Santiago, Adriana. Hospital de Niños “Prof Dr Ramón Exeni”; Argentina
dc.description.fil
Fil: Exeni, Ramón Alfonso. Hospital de Niños “Prof Dr Ramón Exeni”; Argentina
dc.description.fil
Fil: Palermo, Marina Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
dc.journal.title
Pediatric Nephrology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1007/s00467-022-05671-6
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