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dc.contributor.author
Marcelino, Virginia
dc.contributor.author
Saracho, Hayde
dc.contributor.author
Kurth, Daniel German
dc.date.available
2023-09-06T10:03:58Z
dc.date.issued
2022
dc.identifier.citation
Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde; LVIII Congreso Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; Mendoza; Argentina; 2022; 1-2
dc.identifier.uri
http://hdl.handle.net/11336/210635
dc.description.abstract
The Argentine Puna represents a unique environment, characterized by high UV radiation, low oxygen pressure and extreme temperature fluctuations. There we find saline water deposits such as Laguna Verde (Salar de Antofalla, 3300 m.a.s.l.) surrounded by large extensions of white gypsum-halite precipitating crusts. These crusts harbor microbial communities distributed in layers (microbial mats) defined by physicochemical requirements, light and oxygen. These mats colonize both solid and sedimentary surfaces. Metagenomic, physiological and geochemical studies reveal a series of strategies that allow these communities to survive in hypersaline wetlands by performing photosynthesis and serving as CO2 sinks. The goal of this job was to determine the biodiversity associated with these large evaporitics biofilms surrounding the lake.It was observed that the microorganisms are organized in two layers in the salt crust, an upper yellow layer, and a lower green layer. Samples were taken using a cut off sterile 10-ml syringe, were fixed immediately in RNAlater and transported to the laboratory on ice. Total genomic DNA was isolated from each of the layers using the FastDNA ™ SPIN Kit for Soil (MP Biomedical) and it was sequenced with Illumina HiSeq. Raw data obtained, was uploaded to the European Bioinformatic Archive (ENA). Once uploaded, it was analyzed using the MGnify Annotation Pipeline developed by Dr. Rob Finn’s Team at EMBL-EBI. This pipeline performed the taxonomic annotation of the raw reads. The taxonomic annotation obtained, showed that the orange layer is dominated by oxygenic photoautotrophic bacteria (Cyanobacteria, ca. 65%) followed by anoxygenic photoautotrophs (Proteobacteria, ca. 10%) from the classes Alphaproteobacteria and Gammaproteobacteria and heterotrophic bacteria (Bacteoridetes, ca. 10%). The green layer is dominated by the phyla Proteobacteria, ca. 50% followed by Bacteroidetes, ca. 30% and Cyanobacteria, ca. 20%. In both layers, the phyla Actinobacteria and Euryarchaeota represent at least 1% of the relative abundance.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
METAGENOMICS
dc.subject
SALT CRUST
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SALINE LAKE
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EXTREMOPHILES
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Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-09-04T10:46:27Z
dc.journal.pagination
1-2
dc.journal.pais
Argentina
dc.journal.ciudad
Mendoza
dc.description.fil
Fil: Marcelino, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Saracho, Hayde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.description.fil
Fil: Kurth, Daniel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/abstracts.pdf
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.conicet.rol
Autor
dc.coverage
Nacional
dc.type.subtype
Congreso
dc.description.nombreEvento
LVIII Congreso Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
dc.date.evento
2022-11
dc.description.ciudadEvento
Mendoza
dc.description.paisEvento
Argentina
dc.type.publicacion
Book
dc.description.institucionOrganizadora
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
dc.source.libro
Libro de Resumenes: LVIII Congreso Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular
dc.type
Congreso
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