Mostrar el registro sencillo del ítem

dc.contributor.author
Marcelino, Virginia  
dc.contributor.author
Saracho, Hayde  
dc.contributor.author
Kurth, Daniel German  
dc.date.available
2023-09-06T10:03:58Z  
dc.date.issued
2022  
dc.identifier.citation
Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde; LVIII Congreso Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular; Mendoza; Argentina; 2022; 1-2  
dc.identifier.uri
http://hdl.handle.net/11336/210635  
dc.description.abstract
The Argentine Puna represents a unique environment, characterized by high UV radiation, low oxygen pressure and extreme temperature fluctuations. There we find saline water deposits such as Laguna Verde (Salar de Antofalla, 3300 m.a.s.l.) surrounded by large extensions of white gypsum-halite precipitating crusts. These crusts harbor microbial communities distributed in layers (microbial mats) defined by physicochemical requirements, light and oxygen. These mats colonize both solid and sedimentary surfaces. Metagenomic, physiological and geochemical studies reveal a series of strategies that allow these communities to survive in hypersaline wetlands by performing photosynthesis and serving as CO2 sinks. The goal of this job was to determine the biodiversity associated with these large evaporitics biofilms surrounding the lake.It was observed that the microorganisms are organized in two layers in the salt crust, an upper yellow layer, and a lower green layer. Samples were taken using a cut off sterile 10-ml syringe, were fixed immediately in RNAlater and transported to the laboratory on ice. Total genomic DNA was isolated from each of the layers using the FastDNA ™ SPIN Kit for Soil (MP Biomedical) and it was sequenced with Illumina HiSeq. Raw data obtained, was uploaded to the European Bioinformatic Archive (ENA). Once uploaded, it was analyzed using the MGnify Annotation Pipeline developed by Dr. Rob Finn’s Team at EMBL-EBI. This pipeline performed the taxonomic annotation of the raw reads. The taxonomic annotation obtained, showed that the orange layer is dominated by oxygenic photoautotrophic bacteria (Cyanobacteria, ca. 65%) followed by anoxygenic photoautotrophs (Proteobacteria, ca. 10%) from the classes Alphaproteobacteria and Gammaproteobacteria and heterotrophic bacteria (Bacteoridetes, ca. 10%). The green layer is dominated by the phyla Proteobacteria, ca. 50% followed by Bacteroidetes, ca. 30% and Cyanobacteria, ca. 20%. In both layers, the phyla Actinobacteria and Euryarchaeota represent at least 1% of the relative abundance.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
METAGENOMICS  
dc.subject
SALT CRUST  
dc.subject
SALINE LAKE  
dc.subject
EXTREMOPHILES  
dc.subject.classification
Biología Celular, Microbiología  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.subject.classification
Bioquímica y Biología Molecular  
dc.subject.classification
Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
Taxonomic analysis of white gypsum-halite precipitations from Laguna Verde  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2023-09-04T10:46:27Z  
dc.journal.pagination
1-2  
dc.journal.pais
Argentina  
dc.journal.ciudad
Mendoza  
dc.description.fil
Fil: Marcelino, Virginia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Saracho, Hayde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.description.fil
Fil: Kurth, Daniel German. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/abstracts.pdf  
dc.conicet.rol
Autor  
dc.conicet.rol
Autor  
dc.conicet.rol
Autor  
dc.coverage
Nacional  
dc.type.subtype
Congreso  
dc.description.nombreEvento
LVIII Congreso Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular  
dc.date.evento
2022-11  
dc.description.ciudadEvento
Mendoza  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Book  
dc.description.institucionOrganizadora
Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular  
dc.source.libro
Libro de Resumenes: LVIII Congreso Anual de la Sociedad Argentina de Investigaciones en Bioquímica y Biología Molecular  
dc.type
Congreso