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dc.contributor.author
Ruiz, María Julia
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Colello, Rocío
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Padola, Nora Lía
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Etcheverría, Analía Inés
dc.date.available
2023-09-01T13:41:17Z
dc.date.issued
2015
dc.identifier.citation
Evaluation of the inhibitory activity of shiga like toxin producing Escherichia coli by Lactobacillus spp. isolated from pigs; 9th Internacional symposium on Shiga toxin (Verocytotoxin) producing Escherichia coli infection; Boston; Estados Unidos; 2015; 10-10
dc.identifier.uri
http://hdl.handle.net/11336/210148
dc.description.abstract
Introduction: Foodborne diseases represent health and economic problems of relevance in the world. Symptoms vary depending on the causal agent, the dose of intake and the age of the group affected. Bacteria most implicated are Shiga toxinproducing Escherichia coli (STEC). To reduce the risk of infection by pathogen is necessary a strict microbiological monitoring throughout the food production chain. One strategy is the use of biopreservation systems as lactic acid bacteria or their metabolites. The genus Lactobacillus is of interest as biotechnological applications and by their probiotic properties. Usually, these strains are isolated from humans because of their greater ability to adhere to and colonize the intestinal epithelium. However studies have shown that strains of Lactobacillus isolated from animals also have beneficial effects on human. Through this study, we determinated the inhibitory activity of Lactobacillus spp. isolated from pigs on EDL933 STEC reference strain. Methods: Samples were taken by swabs from pig, carcasses and pork cuts and incubated in MRS broth at 37ºC for 48h. Forty five percent of the strains were characterized by biochemical tests as lactobacilli. These strains were individually inoculated on MRS agar plates and incubated at 37ºC for 48h, then they were exposed to chloroform vapors. Then the plates were overlaid with LB agar containing E. coli EDL933 (106 ufc/ml) and incubated overnight at 37 ºC. Finally, the inhibitory activity was evaluated by observing the translucent halo around the colony. Results and discussion: Sixty three percent of the strains developed a halo greater than 1 mm. This indicates that the Lactobacillus have antagonistic properties against this pathogen. Implications: These results show that lactic acid bacteria strains isolated from pigs can become a useful biotechnological tool for controlling E. coli O157:H7, it generates alternatives for producing foods naturally protected and improve the microbiological quality in the food industry.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Ohio State University
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Inhibitory activity
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Lactobacillus spp.
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Pigs
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Otras Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Evaluation of the inhibitory activity of shiga like toxin producing Escherichia coli by Lactobacillus spp. isolated from pigs
dc.type
info:eu-repo/semantics/publishedVersion
dc.type
info:eu-repo/semantics/conferenceObject
dc.type
info:ar-repo/semantics/documento de conferencia
dc.date.updated
2023-01-26T17:43:53Z
dc.journal.pagination
10-10
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Ohio
dc.description.fil
Fil: Ruiz, María Julia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
dc.description.fil
Fil: Colello, Rocío. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
dc.description.fil
Fil: Padola, Nora Lía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
dc.description.fil
Fil: Etcheverría, Analía Inés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
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Autor
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Autor
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Autor
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Autor
dc.coverage
Internacional
dc.type.subtype
Simposio
dc.description.nombreEvento
9th Internacional symposium on Shiga toxin (Verocytotoxin) producing Escherichia coli infection
dc.date.evento
2015-09-13
dc.description.ciudadEvento
Boston
dc.description.paisEvento
Estados Unidos
dc.type.publicacion
Book
dc.description.institucionOrganizadora
Ohio State University
dc.source.libro
Oral abstracts & Poster abstracts: 9th International Symposium On Shiga Toxin (Verocytotoxin) Producing Escherichia coli Infections
dc.date.eventoHasta
2015-09-16
dc.type
Simposio
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