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dc.contributor.author
Liscano, Yamil  
dc.contributor.author
Medina, Laura  
dc.contributor.author
Oñate Garzón, Jose  
dc.contributor.author
Gúzman, Fanny  
dc.contributor.author
Pickholz, Mónica Andrea  
dc.contributor.author
Delgado, Jean Paul  
dc.date.available
2023-08-30T19:24:19Z  
dc.date.issued
2021-04  
dc.identifier.citation
Liscano, Yamil; Medina, Laura; Oñate Garzón, Jose; Gúzman, Fanny; Pickholz, Mónica Andrea; et al.; In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax); Multidisciplinary Digital Publishing Institute; Pharmaceutics; 13; 4; 4-2021; 1-32  
dc.identifier.uri
http://hdl.handle.net/11336/210003  
dc.description.abstract
In order to combat bacterial and cancer resistance, we identified peptides (pugnins) with dual antibacterial l–anticancer activity from the Boana pugnax (B. pugnax) skin transcriptome through in silico analysis. Pugnins A and B were selected owing to their high similarity to the DS4.3 peptide, which served as a template for their alignment to the B. pugnax transcriptome, as well as their function as part of a voltage-dependent potassium channel protein. The secondary peptide structure stability in aqueous medium was evaluated as well, and after interaction with the Escherichia coli (E. coli) membrane model using molecular dynamics. These pugnins were synthesized via solid-phase synthesis strategy and verified by Reverse phase high-performance liquid chromatography (RP-HPLC) and mass spectrometry. Subsequently, their alpha-helix structure was determined by circular dichroism, after which antibacterial tests were then performed to evaluate their antimicrobial activity. Cytotoxicity tests against cancer cells also showed selectivity of pugnin A toward breast cancer (MFC7) cells, and pugnin B toward prostate cancer (PC3) cells. Alternatively, flow cytometry revealed necrotic cell damage with a major cytotoxic effect on human keratinocytes (HaCaT) control cells. Therefore, the pugnins found in the transcriptome of B. pugnax present dual antibacterial– anticancer activity with reduced selectivity to normal eukaryotic cells.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Multidisciplinary Digital Publishing Institute  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/  
dc.subject
ANTICANCER  
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ANTIMICROBIAL  
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BOANA PUGNAX  
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PEPTIDE  
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PUGNIN  
dc.subject.classification
Biofísica  
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Ciencias Biológicas  
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS  
dc.title
In silico selection and evaluation of pugnins with antibacterial and anticancer activity using skin transcriptome of treefrog (Boana pugnax)  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2023-08-30T10:47:03Z  
dc.identifier.eissn
1999-4923  
dc.journal.volume
13  
dc.journal.number
4  
dc.journal.pagination
1-32  
dc.journal.pais
Suiza  
dc.journal.ciudad
Lausana  
dc.description.fil
Fil: Liscano, Yamil. Universidad Santiago de Cali; Colombia. Universidad de Antioquia; Colombia  
dc.description.fil
Fil: Medina, Laura. Universidad de Antioquia; Colombia  
dc.description.fil
Fil: Oñate Garzón, Jose. Universidad Santiago de Cali; Colombia  
dc.description.fil
Fil: Gúzman, Fanny. Pontificia Universidad Católica de Valparaíso; Chile  
dc.description.fil
Fil: Pickholz, Mónica Andrea. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Física; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Ciudad Universitaria. Instituto de Física de Buenos Aires. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Física de Buenos Aires; Argentina  
dc.description.fil
Fil: Delgado, Jean Paul. Universidad de Antioquia; Colombia  
dc.journal.title
Pharmaceutics  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1999-4923/13/4/578  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/pharmaceutics13040578