Harder, better, faster, stronger: Frost tolerance of Eucalyptus benthamii under cold acclimation

Abstract

Eucalypts are the most planted hardwood trees worldwide because of their very rapid growth, exceptional wood quality and adaptability. However, most commercial species and derived hybrids are sensitive to frost, which remains as the largest obstacle to their introduction in warm/temperate climates. As evergreen species, Eucalypts have developed the ability to tolerate frost events based on physiological and molecular responses triggered by previous exposure to cold temperatures, globally named cold acclimation. To characterize the acclimation process in two species with different tolerance to frost, E. grandis (Eg) and E. benthamii (Eb), seedlings were exposed for different times to low temperatures. Frost tolerance was estimated in leaves by an electrolyte leakage assay, and metabolome and morpho-physiological changes studied and correlated to the observed acclimation responses. Eb showed higher basal frost tolerance and an earlier and stronger acclimation response to cold temperatures than in the frost sensitive Eg. Eb was able to modify several morpho-physiological parameters, with a restriction in plant height, leaf area and leaf fresh weight during acclimation. Metabolome characterization allowed us to differentiate species and strengthen our understanding of their acclimation response dynamics. Interestingly, Eb displayed an early phase of sugar accumulation followed by a rise of different metabolites with possible roles as osmolytes and antioxidants, that correlated to frost tolerance and may explain Eb higher capacity to acclimate. This novel approach has helped us to point to the main metabolic processes underlying the cold tolerance acquisition process in two relevant Eucalyptus species.