Artículo
Membrane retrieval after Immediately Releasable Pool (IRP) exocytosis is produced by dynamin-dependent and dynamin-independent/protein kinase C-dependent mechanisms
Bayonés, Lucas
; Alfonso Bueno, Samuel Alberto
; Montenegro, Mauricio Norman
; Moya Diaz, José Abelino
; Caspe, Octavio
; Gallo, Luciana Ines
; Marengo, Fernando Diego
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Fecha de publicación:
10/2022
Editorial:
Wiley Blackwell Publishing, Inc
Revista:
Journal of Neurochemistry
ISSN:
0022-3042
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
The importance of the immediately releasable pool (IRP) of vesicles was proposed to reside in the maintenance of chromaffin cell secretion during the firing of action potentials at basal physiological frequencies. To accomplish this duty, IRP should be replenished as a function of time. We have previously reported that an action potential-like stimulus (APls) triggers the release of ~50% IRP, followed by a fast dynamin-dependent endocytosis and an associated rapid replenishment process. In this work, we investigated the endocytosis and IRP replenishment produced after the exocytosis of variable IRP fractions in mice primary chromaffin cell cultures. Exocytosis and endocytosis were estimated by membrane capacitance measurements obtained in patch-clamped cells. In addition to the dynamin-dependent fast endocytosis activated after the application of APls or 5 ms squared depolarizations, we found that depolarizations lasting 25–50 ms, which release >80% of IRP, are related with a fast dynamin-independent, Ca2+- and protein kinase C (PKC)-dependent endocytosis (time constant <1 s). PKC inhibitors, such as staurosporine, bisindolylmaleimide XI, PKC 19–31 peptide, and prolonged treatments with high concentrations of phorbol esters, reduced and decelerated this endocytosis. Additionally, we found that the inhibition of PKC also abolished a slow component of replenishment (time constant ~8 s) observed after total IRP exocytosis. Therefore, our results suggest that PKC contributes to the coordination of membrane retrieval and vesicle replenishment mechanisms that occur after the complete exocytosis of IRP. (Figure presented.).
Palabras clave:
CALCIUM
,
PROTEIN KINASE C
,
SECRETION
,
SECRETORY VESICLE
,
VESICLE RECYCLING
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Identificadores
Colecciones
Articulos(IFIBYNE)
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Articulos de INST.DE FISIOL., BIOL.MOLECULAR Y NEUROCIENCIAS
Citación
Bayonés, Lucas; Alfonso Bueno, Samuel Alberto; Montenegro, Mauricio Norman; Moya Diaz, José Abelino; Caspe, Octavio; et al.; Membrane retrieval after Immediately Releasable Pool (IRP) exocytosis is produced by dynamin-dependent and dynamin-independent/protein kinase C-dependent mechanisms; Wiley Blackwell Publishing, Inc; Journal of Neurochemistry; 163; 5; 10-2022; 391-405
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