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dc.contributor.author
Mazzucco Panizza, Matilde
dc.contributor.author
Cutullé, Christian
dc.contributor.author
Primo, Maria Evangelina
dc.contributor.author
Morel, Facundo Nicolás
dc.contributor.author
Sebastian, Patrick Stephan
dc.contributor.author
Nava, Santiago
dc.date.available
2023-08-28T15:07:48Z
dc.date.issued
2022-11
dc.identifier.citation
Mazzucco Panizza, Matilde; Cutullé, Christian; Primo, Maria Evangelina; Morel, Facundo Nicolás; Sebastian, Patrick Stephan; et al.; Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus; Elsevier Science; Veterinary Parasitology; 311; 11-2022; 1-6
dc.identifier.issn
0304-4017
dc.identifier.uri
http://hdl.handle.net/11336/209577
dc.description.abstract
The aim of this study was to evaluate the vectorial competence of Rhipicephalus microplus to transmit Anaplasma marginale transovarially, by analyzing the results of three different but complementary assays. First, larvae of R. microplus were fed on a calf infected with the isolate S1P of A. marginale. The engorged females obtained were analyzed by PCR and incubated for oviposition. After hatching, larvae were analyzed by PCR and fed on susceptible splenectomized cattle. Although A. marginale was detected in the females, no A. marginale DNA was amplified from the larvae and transmission of A. marginale to cattle was not recorded. In the second experiment, R. microplus larvae were fed on cattle naturally infected with field isolates of A. marginale and experimentally infected with the isolate S1P of A. marginale. After detachment, engorged females were incubated for oviposition. The offspring were analyzed by PCR, with negative results. Finally, free-living larvae of R. microplus collected from pasture on farms with cattle infected with A. marginale were analyzed by PCR for Anaplasma infection. All samples analyzed were negative for A. marginale. The results of this work indicate that transovarial transmission of A. marginale by R. microplus is unlikely to occur.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier Science
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
ANAPLASMA MARGINALE
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ANAPLASMOSIS
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CATTLE
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RHIPICEPHALUS MICROPLUS
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TRANSOVARIAL TRANSMISSION
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Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Assays to evaluate the transovarial transmission of Anaplasma marginale by Rhipicephalus microplus
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-08-08T13:39:07Z
dc.journal.volume
311
dc.journal.pagination
1-6
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Mazzucco Panizza, Matilde. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
dc.description.fil
Fil: Cutullé, Christian. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
dc.description.fil
Fil: Primo, Maria Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
dc.description.fil
Fil: Morel, Facundo Nicolás. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
dc.description.fil
Fil: Sebastian, Patrick Stephan. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
dc.description.fil
Fil: Nava, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina
dc.journal.title
Veterinary Parasitology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0304401722001625
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.vetpar.2022.109808
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