Repositorio Institucional
Repositorio Institucional
CONICET Digital
  • Inicio
  • EXPLORAR
    • AUTORES
    • DISCIPLINAS
    • COMUNIDADES
  • Estadísticas
  • Novedades
    • Noticias
    • Boletines
  • Ayuda
    • General
    • Datos de investigación
  • Acerca de
    • CONICET Digital
    • Equipo
    • Red Federal
  • Contacto
JavaScript is disabled for your browser. Some features of this site may not work without it.
  • INFORMACIÓN GENERAL
  • RESUMEN
  • ESTADISTICAS
 
Artículo

Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products

Ortiz, Gastón EzequielIcon ; Guitart, María Eugenia; Cavalitto, Sebastian FernandoIcon ; Alberto, Edgardo OmarIcon ; Fernandez Lahore, Marcelo; Blasco, MartínIcon
Fecha de publicación: 08/2015
Editorial: Springer
Revista: Bioprocess And Biosystems Engineering
ISSN: 1615-7591
e-ISSN: 1615-7605
Idioma: Inglés
Tipo de recurso: Artículo publicado
Clasificación temática:
Bioprocesamiento Tecnológico, Biocatálisis, Fermentación

Resumen

The application of cellulases in saccharification processes is restricted by its production cost. Consequently, new fungal strains able to elaborate higher cellulases titers and with special activity profiles are required to make the process economical. The aim of this investigation was to find a promising wild-type Trichoderma strain for cellulases production. The Trichoderma reesei strain 938 (CBS 836.91) was selected among twenty strains on the basis of cellulase-agar-plate screening. Evaluation of the selected strain on six solid substrates indicated the highest activities to be obtained from wheat bran. Statistical analyses of the experimental design indicated a significant effect of pH and moisture on the generation of endoglucanase (EGA) and filter-paper (FPA) activity. Furthermore, a central-composite design-based optimization revealed that pH values between 6.4 and 6.6 and moisture from 74 to 94 % were optimal for cellulases production. Under these conditions, 8?10 IU gds-1 of FPA and 15.6?17.8 IU gds-1 of EGA were obtained. In addition, cultivation in a rotating-drum reactor under optimal conditions gave 8.2 IU gds-1 FPA and 13.5 IU gds-1 EGA. Biochemical characterization of T. reesei 938 cellulases indicated a substantially higher resistance to 4 mM Fe?2 and a slightly greater tolerance to alkaline pH in comparison to Celluclast. These results suggest that T. reesei 938 could be a promising candidate for improved cellulases production through direct-evolution strategies.
Palabras clave: Trichoderma Reesei , Cellulases Production , Solid-State Fermentation , Drum Bioreactor , Design of Experiments
Ver el registro completo
 
Archivos asociados
Thumbnail
 
Tamaño: 1.363Mb
Formato: PDF
.
Descargar
Licencia
info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)
Identificadores
URI: http://hdl.handle.net/11336/20937
DOI: http://dx.doi.org/10.1007/s00449-015-1451-2
URL: https://link.springer.com/article/10.1007%2Fs00449-015-1451-2
Colecciones
Articulos(CCT - LA PLATA)
Articulos de CTRO.CIENTIFICO TECNOL.CONICET - LA PLATA
Articulos(CINDEFI)
Articulos de CENT.DE INV EN FERMENTACIONES INDUSTRIALES (I)
Articulos(IIB-INTECH)
Articulos de INST.DE INVEST.BIOTECNOLOGICAS - INSTITUTO TECNOLOGICO CHASCOMUS
Citación
Ortiz, Gastón Ezequiel; Guitart, María Eugenia; Cavalitto, Sebastian Fernando; Alberto, Edgardo Omar; Fernandez Lahore, Marcelo; et al.; Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products; Springer; Bioprocess And Biosystems Engineering; 38; 11; 8-2015; 2117-2128
Compartir
Altmétricas
 

Enviar por e-mail
Separar cada destinatario (hasta 5) con punto y coma.
  • Facebook
  • X Conicet Digital
  • Instagram
  • YouTube
  • Sound Cloud
  • LinkedIn

Los contenidos del CONICET están licenciados bajo Creative Commons Reconocimiento 2.5 Argentina License

https://www.conicet.gov.ar/ - CONICET

Inicio

Explorar

  • Autores
  • Disciplinas
  • Comunidades

Estadísticas

Novedades

  • Noticias
  • Boletines

Ayuda

Acerca de

  • CONICET Digital
  • Equipo
  • Red Federal

Contacto

Godoy Cruz 2290 (C1425FQB) CABA – República Argentina – Tel: +5411 4899-5400 repositorio@conicet.gov.ar
TÉRMINOS Y CONDICIONES