Molecular detection of Mycobacterium tuberculosis complex in a captive aguará popé (Procyon cancrivorus) with macroscopic tuberculosis like-lesions

Abstract

Tuberculosis is a chronic and contagious infectious disease caused by multi-host species of the genus Mycobacterium grouped within the Mycobacterium tuberculosis complex. These pathogenic bacteria mainly affect mammals, including humans. The most recognized species is Mycobacterium bovis, the causative agent of bovine tuberculosis in livestock. Although livestock is the main host of M. bovis, this species is frequently isolated from wild animals. Wild native mammals from Central and South America, as the crab-eating raccoon or “aguará popé” (Procyon cancrivorus), may act as a source of tuberculosis and may represent a human health risk, especially in captive scenarios, due to closer animal-human interaction. However, the only presence of infection in wild animals is not enough to determine their epidemiological role in the disease. Here we identify tuberculosis in a captive aguará popé with clinical signs and lung macroscopic tuberculosis-like lesions during necropsy. We detected tuberculosis by polymerase chain reaction assay. DNA was extracted directly from lung tissue and the amplification target was the insertion sequence 6110. This study contributes to investigate the presence of the disease in wild native animals of Argentina and supports the knowledge that wild mammals may act as a source of TB for humans and domestic animals.