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dc.contributor.author
Cabeza, Juan P.
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Cámera, Juan
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Briski, Olinda
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Yauri Felipe, Minerva
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Salamone, Daniel Felipe
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Gambini, Andres
dc.date.available
2023-08-22T10:09:09Z
dc.date.issued
2022-12-16
dc.identifier.citation
Cabeza, Juan P.; Cámera, Juan; Briski, Olinda; Yauri Felipe, Minerva; Salamone, Daniel Felipe; et al.; Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation; Multidisciplinary Digital Publishing Institute; Animals; 12; 24; 16-12-2022; 1-12
dc.identifier.issn
2076-2615
dc.identifier.uri
http://hdl.handle.net/11336/208812
dc.description.abstract
After sperm-oocyte fusion, intracytoplasmic rises of calcium (Ca) induce the release of zinc (Zn) out of the oocyte (Zn sparks). Both phenomena are known to play an essential role in the oocyte activation process. Our work aimed to explore different protocols for activating bovine and porcine oocytes using the novel zinc chelator 1,10-phenanthroline (PHEN) and to compare developmental rates and quality to bovine IVF and parthenogenetic ionomycin-induced embryos in both species. Different incubation conditions for the zinc chelator were tested, including its combination with ionomycin. Embryo quality was assessed by immunofluorescence of SOX2, SOX17, OCT4, and CDX2 and total cell number at the blastocyst stage. Even though blastocyst development was achieved using a zinc chelator in bovine, bypassing calcium oscillations, developmental rates, and blastocyst quality were compromised compared to embryos generated with sperm-induced or ionomycin calcium rise. On the contrary, zinc chelation is sufficient to trigger oocyte activation in porcine. Additionally, we determined the optimal exposure to PHEN for this species. Zinc chelation and artificial induction of calcium rise combined did not improve developmental competence. Our results contribute to understanding the role of zinc during oocyte activation and preimplantation embryo development across different mammalian species.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Multidisciplinary Digital Publishing Institute
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
ASSISTED OOCYTE ACTIVATION
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CALCIUM OSCILLATION
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EMBRYO DEVELOPMENT
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OOCYTE TO EMBRYO TRANSITION
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ZINC CHELATOR
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Otras Ciencias Biológicas
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Preimplantation Developmental Competence of Bovine and Porcine Oocytes Activated by Zinc Chelation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-08T00:23:00Z
dc.journal.volume
12
dc.journal.number
24
dc.journal.pagination
1-12
dc.journal.pais
Estados Unidos
dc.description.fil
Fil: Cabeza, Juan P.. Universidad de Buenos Aires; Argentina
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Fil: Cámera, Juan. Universidad de Buenos Aires; Argentina
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Fil: Briski, Olinda. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
dc.description.fil
Fil: Yauri Felipe, Minerva. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
dc.description.fil
Fil: Salamone, Daniel Felipe. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Unidad Ejecutora de Investigaciones en Producción Animal. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Unidad Ejecutora de Investigaciones en Producción Animal; Argentina
dc.description.fil
Fil: Gambini, Andres. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires; Argentina. University of Queensland; Australia
dc.journal.title
Animals
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/ani12243560
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2076-2615/12/24/3560
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