Artículo
Vegetative forms of Clostridium chauvoei trigger apoptotic and inflammatory responses on macrophages
Cáceres, Claudia Soledad; Gallo, Giovanna Lucrecia
; Colocho, Florencia Amancay
; Silva, Juan Eduardo
; Garay, Juan Agustín; Mattar Domínguez, María Aída
Fecha de publicación:
11/2022
Editorial:
Elsevier
Revista:
Anaerobe
ISSN:
1075-9964
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background: Clostridium chauvoei is a gram-positive, spore-forming, strictly anaerobic bacterium that causes blackleg, a disease that affects cattle by inducing fulminant myonecrosis, thereby leading to high and constant losses of cattle. Macrophages (Mɸs) are depleted in tissues infected with the vegetative form of C. chauvoei, but the mechanism remains partially known. Consequently, Mɸs may be a critical target in the pathogenicity of C. chauvoei. Aim: The objective of this work was to study the mechanism of death of mouse-primary Mɸs infected in vitro for 24 h with the vegetative form of C. chauvoei. Methods: Mouse peritoneal Mɸs were infected in vitro with different multiplicities of infection (MOIs) of C. chauvoei (i.e., 5:1, 20:1, and 100:1). After 24 h post-infection, cell viability (MTT reduction assay), apoptosis (apoptotic bodies, DNA ladder, and Annexin V assays), and inflammatory cell response (iNOS and TNF-α expression) were assessed. Results: All the MOIs investigated decreased cell viability. An MOI of 20:1 caused the highest production of apoptotic bodies and an electrophoretic DNA-ladder pattern typical of an apoptosis cell death process. These results were corroborated using the Annexin V-flow cytometry assay. Concurrently with apoptotic cell death, Mφs expressed iNOS and TNF-α. Conclusion: Inflammation-mediated apoptosis of Mφs can be a potential mechanism of evasion of the immune response used by C. chauvoei in tissues for depleting phagocytic cells at the site of infection.
Palabras clave:
APOPTOSIS
,
CLOSTRIDIUM CHAUVOEI
,
IN VITRO
,
INFLAMMATION
,
MACROPHAGE
Archivos asociados
Licencia
Identificadores
Colecciones
Articulos (CEVHAN)
Articulos de CENTRO DE VIROLOGIA HUMANA Y ANIMAL
Articulos de CENTRO DE VIROLOGIA HUMANA Y ANIMAL
Articulos(IMIBIO-SL)
Articulos de INST. MULTIDICIPLINARIO DE INV. BIO. DE SAN LUIS
Articulos de INST. MULTIDICIPLINARIO DE INV. BIO. DE SAN LUIS
Citación
Cáceres, Claudia Soledad; Gallo, Giovanna Lucrecia; Colocho, Florencia Amancay; Silva, Juan Eduardo; Garay, Juan Agustín; et al.; Vegetative forms of Clostridium chauvoei trigger apoptotic and inflammatory responses on macrophages; Elsevier; Anaerobe; 77; 102616; 11-2022; 1-5
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