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dc.contributor.author
Losi, Aba
dc.contributor.author
Bonomi, Hernan Ruy
dc.contributor.author
Michael, Norbert
dc.contributor.author
Tang, Kun
dc.contributor.author
Zhao, Kai Hong
dc.date.available
2017-07-18T19:57:47Z
dc.date.issued
2017-05
dc.identifier.citation
Losi, Aba; Bonomi, Hernan Ruy; Michael, Norbert; Tang, Kun; Zhao, Kai Hong; Time-Resolved Energetics of Photoprocesses in Prokaryotic Phytochrome-Related Photoreceptors; American Society for Photobiology; Photochemistry and Photobiology; 93; 3; 5-2017; 733-740
dc.identifier.issn
0031-8655
dc.identifier.uri
http://hdl.handle.net/11336/20858
dc.description.abstract
Time-resolved photoacoustics (PA) is uniquely able to explore the energy landscape of photoactive proteins and concomitantly detects light-induced volumetric changes (ΔV) accompanying the formation and decay of transient species in a time window between ca. 20 ns and 5 μs. Here, we report PA measurements on diverse photochromic bilin-binding photoreceptors of prokaryotic origin: (1) the chromophore-binding GAF3 domain of the red (R)/green (G) switching cyanobacteriochrome 1393 (Slr1393g3) from Synechocystis; (2) the red/far red (R/FR) Synechocystis Cph1 phytochrome; (3) full-length and truncated constructs of Xanthomonas campestris bacteriophytochrome (XccBphP), absorbing up to the NIR spectral region. In almost all cases, photoisomerization results in a large fraction of energy dissipated as heat (up to 90%) on the sub-ns scale, reflecting the low photoisomerization quantum yield (<0.2). This "prompt" step is accompanied by a positive ΔV1 = 5-12.5 mL mol-1 . Formation of the first intermediate is the sole process accessible to PA, with the notable exception of Slr1393g3-G for which ΔV1 = +4.5 mL mol-1 is followed by a time-resolved, energy-conserving contraction ΔV2 = -11.4 mL mol-1 , τ2 = 180 ns at 2.4°C. This peculiarity is possibly due to a larger solvent occupancy of the chromophore cavity for Slr1393g3-G.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Photobiology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Time-Resolved Photoacoustics
dc.subject
Bathy Bacteriophytochrome
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Phytochrome
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Chromophore
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Photoisomerization
dc.subject.classification
Biofísica
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Time-Resolved Energetics of Photoprocesses in Prokaryotic Phytochrome-Related Photoreceptors
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-18T14:51:21Z
dc.identifier.eissn
1751-1097
dc.journal.volume
93
dc.journal.number
3
dc.journal.pagination
733-740
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Lawrence
dc.description.fil
Fil: Losi, Aba. Università di Parma; Italia
dc.description.fil
Fil: Bonomi, Hernan Ruy. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil
Fil: Michael, Norbert. Technishe Universitat Berlin; Alemania
dc.description.fil
Fil: Tang, Kun. Huazhong Agricultural University; China. Max Planck Institute for Chemical Energy Conversion; Alemania
dc.description.fil
Fil: Zhao, Kai Hong. Huazhong Agricultural University; China
dc.journal.title
Photochemistry and Photobiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/php.12728
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1111/php.12728
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