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dc.contributor.author
Gutiérrez Cacciabue, Dolores
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dc.contributor.author
Rajal, Verónica Beatriz
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dc.date.available
2023-08-16T18:53:47Z
dc.date.issued
2022-03
dc.identifier.citation
Gutiérrez Cacciabue, Dolores; Rajal, Verónica Beatriz; Estimating decay kinetic parameters and persistence of bacteria in water is essential for future modelling; Elsevier; Chemical Engineering Research & Design; 179; 3-2022; 175-187
dc.identifier.issn
0263-8762
dc.identifier.uri
http://hdl.handle.net/11336/208506
dc.description.abstract
The aim of this work was to obtain decay kinetic parameters for bacteria sedimentation–resuspension in water. For that, synthetic water matrices prepared with four particle sizes at 2.5 and 5 g/l, were spiked with Escherichia coli and Enterococcus faecalis, selected as Gram-negative and -positive models, respectively. Matrices with bacteria without solids were used as controls. Turbidity was measured and culturable bacteria decay was evaluated using membrane filtration over time. Also, the persistence of culturable E. faecalis and its DNA (detected by real-time PCR) was compared. When no colonies were detected, water matrices were mixed to re-suspend sediments and surface samples were collected and analysed. Spearman test was applied to find correlation between bacteria and turbidity. A persistence coefficient (PC) was defined and several kinetic parameters (decay rate constants, R2, T90) were calculated from experimental data. While culturable E. coli disappeared from the surface of all water matrices with turbidity (p < 0.05), E. faecalis only showed strong and positive correlation in matrices with higher turbidity and smaller particles. E. coli decayed slower when interacting with smallest solids (<44 μm) than with the largest ones (>149 μm), being T90 3–4 times higher in the former. Also, it reappeared and persisted in the surface of all matrices after resuspension despite solid concentration (PC: 0.5−0.9). Instead, culturable E. faecalis, persisted less (lower PC values) in matrices with 2.5 g/l than with 5 g/l and no logic relation was observed for any rate constant with particle size. E. faecalis DNA remained in suspension for longer periods until the end of the experience as seen through their lower rate constants (kDNA < 0.05 h−1). Accurate decay kinetic parameters, like the ones obtained here, are crucial for modelling the fate and transport of bacteria in water and to perform a robust and realistic quantitative risk assessment.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Elsevier
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dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
BACTERIA MODELS
dc.subject
DECAY KINETICS PARAMETERS
dc.subject
PERSISTENCE COEFFICIENT
dc.subject
REAL-TIME PCR
dc.subject
TURBIDITY
dc.subject
WATER QUALITY
dc.subject.classification
Otras Ingeniería del Medio Ambiente
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dc.subject.classification
Ingeniería del Medio Ambiente
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dc.subject.classification
INGENIERÍAS Y TECNOLOGÍAS
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dc.title
Estimating decay kinetic parameters and persistence of bacteria in water is essential for future modelling
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-07T20:19:35Z
dc.journal.volume
179
dc.journal.pagination
175-187
dc.journal.pais
Países Bajos
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dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Gutiérrez Cacciabue, Dolores. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina
dc.description.fil
Fil: Rajal, Verónica Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Salta. Instituto de Investigaciones para la Industria Química. Universidad Nacional de Salta. Facultad de Ingeniería. Instituto de Investigaciones para la Industria Química; Argentina. Nanyang Technological University. Singapore Centre for Environmental Life Sciences Engineering; Singapur
dc.journal.title
Chemical Engineering Research & Design
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dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.cherd.2022.01.014
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0263876222000144
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