Artículo
Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models
Yokobori, Noemí
; López, Beatriz Graciela; Geffner, Laura Judith
; Sabio y García, Carmen Alejandra
; Schierloh, Luis Pablo
; Barrera, Lucía; de la Barrera, Silvia Susana
; Sakai, Shunsuke; Kawamura, Ikuo; Mitsuyama, Maso; Ritacco, Gloria Viviana
; Sasiain, María del Carmen
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Fecha de publicación:
06/2013
Editorial:
Elsevier Science
Revista:
Infection, Genetics and Evolution
ISSN:
1567-1348
Idioma:
Español
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Mycobacterium tuberculosis has a considerable degree of genetic variability resulting in different epidemiology and disease outcomes. We evaluated the pathogen-host cell interaction of two genetically closely-related multidrug-resistant M. tuberculosis strains of the Haarlem family, namely the strain M, responsible for an extensive multidrug-resistant tuberculosis outbreak, and its kin strain 410 which caused a single case in two decades. Intracellular growth and cytokine responses were evaluated in human monocyte-derived macrophages and dU937 macrophage-like cells. In monocyte-derived macrophages, strain M grew more slowly and induced lower levels of TNF-α and IL-10 than 410, contrasting with previous studies with other strains, where a direct correlation was observed between increased intracellular growth and epidemiological success. On the other hand, in dU937 cells, no difference in growth was observed between both strains, and strain M induced significantly higher TNF-α levels than strain 410. We found that both cell models differed critically in the expression of receptors for M. tuberculosis entry, which might explain the different infection outcomes. Our results in monocyte-derived macrophages suggest that strain M relies on a modest replication rate and cytokine induction, keeping a state of quiescence and remaining rather unnoticed by the host. Collectively, our results underscore the impact of M. tuberculosis intra-species variations on the outcome of host cell infection and show that results can differ depending on the in vitro infection model
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Articulos de INST.DE MEDICINA EXPERIMENTAL
Articulos de INST.DE MEDICINA EXPERIMENTAL
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Articulos de SEDE CENTRAL
Citación
Yokobori, Noemí; López, Beatriz Graciela; Geffner, Laura Judith; Sabio y García, Carmen Alejandra; Schierloh, Luis Pablo; et al.; Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models; Elsevier Science; Infection, Genetics and Evolution; 16; 6-2013; 151-156
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