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dc.contributor.author
Cholich, Luciana Andrea  
dc.contributor.author
Pistan, Maria Elena  
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Torres, Ana María  
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Ortega, Hugo Hector  
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Gardner, Dale R.  
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Bustillo, Soledad  
dc.date.available
2023-08-14T12:44:35Z  
dc.date.issued
2020-12  
dc.identifier.citation
Cholich, Luciana Andrea; Pistan, Maria Elena; Torres, Ana María; Ortega, Hugo Hector; Gardner, Dale R.; et al.; Cytotoxic activity induced by the alkaloid extract from Ipomoea carnea on primary murine mixed glial cultures; Pergamon-Elsevier Science Ltd; Toxicon; 188; 12-2020; 134-141  
dc.identifier.issn
0041-0101  
dc.identifier.uri
http://hdl.handle.net/11336/208109  
dc.description.abstract
The prolonged consumption of Ipomoea carnea produces neurologic symptoms in animals and a typical histological lesion, cytoplasmic vacuolization, especially in neurons. The toxic principles of I. carnea are the alkaloids swainsonine and calystegines B1, B2, B3 and C1. In this study, primary brain cultures from newborn mouse containing mixed glial cells were utilized. These cells were exposed to Ipomoea extracts containing between 0 and 250 μM swainsonine for 48 h. Morphological changes were investigated through Phase Contrast microscopy and Rosenfeld's staining. The extract induced cytoplasmic vacuolization in astrocytes and microglia in a dose dependent manner, being more evident when cultures were exposed to 250 μM of swainsonine. In addition, acridine orange staining evidenced an increase in the number of lysosomes in both microglia and astrocytes cells. Consistent with this, scanning electron microscopy also showed that both types of cells presented morphological characteristics of cell activation. Ultrastructurally, cells showed vacuoles filled with amorphous material and surrounded by a single membrane and also multilayer membranes. Taken together, these findings suggest that swainsonine along with calystegines, are probably responsible for the activation of glial cells due to a possible lysosomal dysfunction and therefore intracellular storage. Our results demonstrate that this in vitro glial cell model is a very good alternative to in vivo studies that require several weeks of animal intoxication to observe similar neurotoxic effects.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Pergamon-Elsevier Science Ltd  
dc.rights
info:eu-repo/semantics/openAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/  
dc.subject
ASTROCYTES  
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CYTOTOXICITY  
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IN VITRO  
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IPOMOEA CARNEA  
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LYSOSOMAL STORAGE DISEASE  
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MICROGLIA  
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SWAINSONINE  
dc.subject.classification
Ciencias Veterinarias  
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Ciencias Veterinarias  
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CIENCIAS AGRÍCOLAS  
dc.title
Cytotoxic activity induced by the alkaloid extract from Ipomoea carnea on primary murine mixed glial cultures  
dc.type
info:eu-repo/semantics/article  
dc.type
info:ar-repo/semantics/artículo  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.date.updated
2021-02-10T21:04:16Z  
dc.journal.volume
188  
dc.journal.pagination
134-141  
dc.journal.pais
Países Bajos  
dc.journal.ciudad
Amsterdam  
dc.description.fil
Fil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina  
dc.description.fil
Fil: Pistan, Maria Elena. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina  
dc.description.fil
Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina  
dc.description.fil
Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina  
dc.description.fil
Fil: Gardner, Dale R.. Poisonous Plant Research Laboratory; Estados Unidos  
dc.description.fil
Fil: Bustillo, Soledad. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina  
dc.journal.title
Toxicon  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0041010120304232  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.toxicon.2020.10.019