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dc.contributor.author
Cholich, Luciana Andrea
dc.contributor.author
Pistan, Maria Elena
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Torres, Ana María
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Ortega, Hugo Hector
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Gardner, Dale R.
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Bustillo, Soledad
dc.date.available
2023-08-14T12:44:35Z
dc.date.issued
2020-12
dc.identifier.citation
Cholich, Luciana Andrea; Pistan, Maria Elena; Torres, Ana María; Ortega, Hugo Hector; Gardner, Dale R.; et al.; Cytotoxic activity induced by the alkaloid extract from Ipomoea carnea on primary murine mixed glial cultures; Pergamon-Elsevier Science Ltd; Toxicon; 188; 12-2020; 134-141
dc.identifier.issn
0041-0101
dc.identifier.uri
http://hdl.handle.net/11336/208109
dc.description.abstract
The prolonged consumption of Ipomoea carnea produces neurologic symptoms in animals and a typical histological lesion, cytoplasmic vacuolization, especially in neurons. The toxic principles of I. carnea are the alkaloids swainsonine and calystegines B1, B2, B3 and C1. In this study, primary brain cultures from newborn mouse containing mixed glial cells were utilized. These cells were exposed to Ipomoea extracts containing between 0 and 250 μM swainsonine for 48 h. Morphological changes were investigated through Phase Contrast microscopy and Rosenfeld's staining. The extract induced cytoplasmic vacuolization in astrocytes and microglia in a dose dependent manner, being more evident when cultures were exposed to 250 μM of swainsonine. In addition, acridine orange staining evidenced an increase in the number of lysosomes in both microglia and astrocytes cells. Consistent with this, scanning electron microscopy also showed that both types of cells presented morphological characteristics of cell activation. Ultrastructurally, cells showed vacuoles filled with amorphous material and surrounded by a single membrane and also multilayer membranes. Taken together, these findings suggest that swainsonine along with calystegines, are probably responsible for the activation of glial cells due to a possible lysosomal dysfunction and therefore intracellular storage. Our results demonstrate that this in vitro glial cell model is a very good alternative to in vivo studies that require several weeks of animal intoxication to observe similar neurotoxic effects.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Pergamon-Elsevier Science Ltd
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.subject
ASTROCYTES
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CYTOTOXICITY
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IN VITRO
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IPOMOEA CARNEA
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LYSOSOMAL STORAGE DISEASE
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MICROGLIA
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SWAINSONINE
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Ciencias Veterinarias
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Ciencias Veterinarias
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CIENCIAS AGRÍCOLAS
dc.title
Cytotoxic activity induced by the alkaloid extract from Ipomoea carnea on primary murine mixed glial cultures
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2021-02-10T21:04:16Z
dc.journal.volume
188
dc.journal.pagination
134-141
dc.journal.pais
Países Bajos
dc.journal.ciudad
Amsterdam
dc.description.fil
Fil: Cholich, Luciana Andrea. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina
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Fil: Pistan, Maria Elena. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
dc.description.fil
Fil: Torres, Ana María. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
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Fil: Ortega, Hugo Hector. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina
dc.description.fil
Fil: Gardner, Dale R.. Poisonous Plant Research Laboratory; Estados Unidos
dc.description.fil
Fil: Bustillo, Soledad. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Química Básica y Aplicada del Nordeste Argentino. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina
dc.journal.title
Toxicon
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0041010120304232
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1016/j.toxicon.2020.10.019
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