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dc.contributor.author
Pantaleno, Rosario
dc.contributor.author
Scuffi, Denise
dc.contributor.author
Costa, Alex
dc.contributor.author
Welchen, Elina
dc.contributor.author
Torregrossa, Roberta
dc.contributor.author
Whiteman, Matthew
dc.contributor.author
García Mata, Carlos
dc.date.available
2023-08-11T15:37:51Z
dc.date.issued
2022-12-23
dc.identifier.citation
Pantaleno, Rosario; Scuffi, Denise; Costa, Alex; Welchen, Elina; Torregrossa, Roberta; et al.; Mitochondrial H2S donor AP39 induces stomatal closure by modulating guard cell mitochondrial activity; American Society of Plant Biologist; Plant Physiology; 191; 3; 23-12-2022; 2001-2011
dc.identifier.issn
0032-0889
dc.identifier.uri
http://hdl.handle.net/11336/207946
dc.description.abstract
Hydrogen sulfide (H2S) is a gaseous signaling molecule involved in numerous physiological processes in plants, including gas exchange with the environment through the regulation of stomatal pore width. Guard cells (GCs) are pairs of specialized epidermal cells that delimit stomatal pores and have a higher mitochondrial density and metabolic activity than their neighboring cells. However, there is no clear evidence on the role of mitochondrial activity in stomatal closure induction. In this work, we showed that the mitochondrial-targeted H2S donor AP39 induces stomatal closure in a dose-dependent manner. Experiments using inhibitors of the mitochondrial electron transport chain (mETC) or insertional mutants in cytochrome c (CYTc) indicated that the activity of mitochondrial CYTc and/or complex IV are required for AP39-dependent stomatal closure. By using fluorescent probes and genetically encoded biosensors we reported that AP39 hyperpolarized the mitochondrial inner potential (ΔΨm) and increased cytosolic ATP, cytosolic hydrogen peroxide levels, and oxidation of the glutathione pool in GCs. These findings showed that mitochondrial-targeted H2S donors induce stomatal closure, modulate guard cell mETC activity, the cytosolic energetic and oxidative status, pointing to an interplay between mitochondrial H2S, mitochondrial activity, and stomatal closure.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society of Plant Biologist
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.subject
AP39
dc.subject
H2S DONOR
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CYTC
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STOMATAL CLOSURE
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Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Mitochondrial H2S donor AP39 induces stomatal closure by modulating guard cell mitochondrial activity
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-06T11:23:40Z
dc.identifier.eissn
1532-2548
dc.journal.volume
191
dc.journal.number
3
dc.journal.pagination
2001-2011
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Rockville
dc.description.fil
Fil: Pantaleno, Rosario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Scuffi, Denise. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mar del Plata. Instituto de Investigaciones Biológicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Instituto de Investigaciones Biológicas; Argentina
dc.description.fil
Fil: Costa, Alex. Università degli Studi di Milano; Italia
dc.description.fil
Fil: Welchen, Elina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
dc.description.fil
Fil: Torregrossa, Roberta. University of Exeter Medical School; Reino Unido
dc.description.fil
Fil: Whiteman, Matthew. University of Exeter Medical School; Reino Unido
dc.description.fil
Fil: García Mata, Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.journal.title
Plant Physiology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/plphys/advance-article/doi/10.1093/plphys/kiac591/6957761
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1093/plphys/kiac591
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