Mostrar el registro sencillo del ítem
dc.contributor.author
Fujiwara, Kei
dc.contributor.author
Allison, Robert. D.
dc.contributor.author
Wang, Richard Y.
dc.contributor.author
Baré, Patricia
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.contributor.author
Matsuura, Kentaro
dc.contributor.author
Schechterly, Cathy
dc.contributor.author
Murthy, Krishna
dc.contributor.author
Marincola, Francesco M.
dc.contributor.author
Alter, Harvey J.
dc.date.available
2017-07-17T22:33:28Z
dc.date.issued
2013-02
dc.identifier.citation
Fujiwara, Kei; Allison, Robert. D.; Wang, Richard Y.; Baré, Patricia; Matsuura, Kentaro; et al.; Investigation of residual hepatitis C virus in presumed recovered subjects; Wiley; Hepatology (baltimore, Md.); 57; 2; 2-2013; 483-491
dc.identifier.issn
0270-9139
dc.identifier.uri
http://hdl.handle.net/11336/20790
dc.description.abstract
Recent studies have found hepatitis C virus (HCV) RNA in peripheral blood mononuclear cells (PBMCs) of the majority of presumed recovered subjects. We investigated this unexpected finding using samples from patients whose HCV RNA and anti-HCV status had been serially confirmed. HCV RNA was detected in PBMCs from 66/67 chronic HCV carriers. Subpopulation analysis revealed that the viral load (log copies/10(6) cells) in B cells (4.14 ± 0.71) was higher than in total PBMCs (3.62 ± 0.71, p<0.05), T cells (1.67 ± 0.88, p<0.05), and non-B/T cells (2.48 ± 1.15, p<0.05). HCV negative-strand RNA was not detected in PBMCs from any of 25 chronically infected patients. No residual viral RNA was detected in total PBMCs or plasma of 59 presumed recovered subjects (11 spontaneous, 48 treatment-induced) using nested real-time PCR with a detection limit of 2 copies/µg RNA (from ∼1x10(6) cells). PBMCs from two healthy HCV-negative blood donors became HCV RNA positive, with B-cell predominance, when mixed in vitro with HCV RNA positive plasma, thus passively mimicking cells from chronic HCV carriers. No residual HCV was detected in liver or other tissues from two spontaneously recovered chimpanzees. Conclusion: 1) HCV RNA was detected in PBMCs of most chronic HCV carriers and was predominant in the B cell subpopulation; 2) HCV detected in PBMCs was in a non-replicative form; 3) HCV passively adsorbed to PBMCs of healthy controls in vitro becoming indistinguishable from PBMCs of chronic HCV carriers; 4) Residual HCV was not detected in the plasma or PBMCs of any spontaneous or treatment recovered subjects or in chimpanzee liver suggesting that the classic pattern of recovery from HCV infection is generally equivalent to viral eradication.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Wiley
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Hcv
dc.subject
Residual
dc.subject
Recovery
dc.subject.classification
Enfermedades Infecciosas
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
Ciencias de la Salud
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.subject.classification
CIENCIAS MÉDICAS Y DE LA SALUD
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.title
Investigation of residual hepatitis C virus in presumed recovered subjects
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-17T21:13:55Z
dc.identifier.eissn
1527-3350
dc.journal.volume
57
dc.journal.number
2
dc.journal.pagination
483-491
dc.journal.pais
Estados Unidos
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.journal.ciudad
Hoboken
dc.description.fil
Fil: Fujiwara, Kei. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Allison, Robert. D.. National Institutes of Health; Estados Unidos. United States Navy; Estados Unidos
dc.description.fil
Fil: Wang, Richard Y.. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Baré, Patricia. National Institutes of Health; Estados Unidos. Academia Nacional de Medicina de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Matsuura, Kentaro. Nagoya City University; Japón
dc.description.fil
Fil: Schechterly, Cathy. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Murthy, Krishna.
dc.description.fil
Fil: Marincola, Francesco M.. National Institutes of Health; Estados Unidos
dc.description.fil
Fil: Alter, Harvey J.. National Institutes of Health; Estados Unidos
dc.journal.title
Hepatology (baltimore, Md.)
![Se ha confirmado la validez de este valor de autoridad por un usuario](/themes/CONICETDigital/images/authority_control/invisible.gif)
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1002/hep.25921/abstract
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1002/hep.25921
Archivos asociados