Artículo
Interleukin-10 controls human peripheral PMN activation triggered by lipopolysaccharide
Martire Greco, Daiana
; Rodriguez Rodrigues, Nahuel Emiliano
; Landoni, Verónica Inés
; Rearte, María Bárbara
; Isturiz, Martín Amadeo
; Fernández, Gabriela Cristina
Fecha de publicación:
06/2013
Editorial:
Elsevier
Revista:
Cytokine
ISSN:
1043-4666
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Large amounts of anti-inflammatory mediators, such as interleukin (IL)-10, are produced and found early in the course of sepsis. We explore the role of IL-10 on neutrophil (PMN) activation/function using an in vitro model. Isolated human PMN were pre-incubated with polysaccharide (LPS) and/or IL-10 for 18 h. Subsequently, a second LPS exposure was performed and CD11b and CD66b up-regulation, and the reactive oxygen species (ROS) generation were measured 2 h later. We found that IL-10 prevented PMN activation and the secretion of TNF-a and IL-8 induced by the first LPS contact. In the absence of IL-10, a second LPS exposure induced additive effects that were prevented by IL-10. Only ROS generation was highly affected by the blockade of PMN-secreted TNF-a or IL-8. Additionally, IL-10 prevented other possible mechanisms of LPS priming. Therefore, IL-10 modulates PMN activation preventing autocrine activating loops and priming mechanisms, rendering PMN less responsive to a second LPS exposure.
Palabras clave:
Lps
,
Interleuquina-10
,
Neutrophils
,
Activacion
,
Il-10
,
In Vitro
,
Septis
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Articulos(IMEX)
Articulos de INST.DE MEDICINA EXPERIMENTAL
Articulos de INST.DE MEDICINA EXPERIMENTAL
Citación
Martire Greco, Daiana; Rodriguez Rodrigues, Nahuel Emiliano; Landoni, Verónica Inés; Rearte, María Bárbara; Isturiz, Martín Amadeo; et al.; Interleukin-10 controls human peripheral PMN activation triggered by lipopolysaccharide; Elsevier; Cytokine; 62; 3; 6-2013; 426-432
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