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dc.contributor.author
Ortiz, María Eugenia
dc.contributor.author
Arias Torres, Ana Josefina
dc.contributor.author
Zelarayán, Liliana Isabel
dc.date.available
2017-07-17T21:09:38Z
dc.date.issued
2015-08
dc.identifier.citation
Ortiz, María Eugenia; Arias Torres, Ana Josefina; Zelarayán, Liliana Isabel; Role of arachidonic acid cascade in Rhinella arenarum oocyte maturation; Cambridge University Press; Zygote; 23; 4; 8-2015; 603-614
dc.identifier.issn
0967-1994
dc.identifier.uri
http://hdl.handle.net/11336/20764
dc.description.abstract
There are no studies that document the production of prostaglandins (PGs) or their role in Rhinella arenarum oocyte maturation. In this study, we analysed the effect of arachidonic acid (AA) andprostaglandins (PGs) on maturation, activation and pronuclear formation in R. arenarum oocytes. Our results demonstrated that AA was capable of inducing maturation in time-dependent and dose-dependent manner. Arachidonic acid-induced maturation was inhibited by indomethacin. PGs from AA hydrolysis, such as prostaglandin F2a (PGF2a) and, to a lesser extent, PGE2, induced meiosis resumption. Oocyte maturation in response to PGF2a was similar to that produced by progesterone (P4). Oocyte response to PGE1 was scarce. Rhinella arenarum oocyte PGF2a-induced maturation showed seasonal variation. From February to June, oocytes presented low sensitivity to PGF2a. In following periods, this response increased until a maximum was reached during October to January, a close temporal correlation with oocyte response to P4 being observed. The effect of PGF2a on maturation was verified by analysing the capacity of oocytes to activate and form pronuclei after being injected with homologous sperm. The cytological analysis of activated oocytes demonstrated the absence of cortical granules in oocytes, suggesting that PGF2a induces germinal vesicle breakdown (GVBD) and meiosis resumption up to metaphase II. In turn, oocytes matured by the action of PGF2a were able to form pronuclei after fertilization in a similar way to oocyte maturated by P4. In microinjection of mature cytoplasm experiments, the transformation of pre-maturation promoting factor (pre-MPF) to MPF was observed when oocytes were treated with PGF2a. In summary, our results illustrated the participation of the AA cascade and its metabolites in maturation, activation and pronuclei formation in R. arenarum.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cambridge University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Arachidonic Acid Cascade
dc.subject
Oocyte Maturation
dc.subject
Prostaglandins
dc.subject
Rhinella Arenarum
dc.subject.classification
Otros Tópicos Biológicos
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Role of arachidonic acid cascade in Rhinella arenarum oocyte maturation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-04T14:09:10Z
dc.identifier.eissn
1469-8730
dc.journal.volume
23
dc.journal.number
4
dc.journal.pagination
603-614
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Ortiz, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.description.fil
Fil: Arias Torres, Ana Josefina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
dc.description.fil
Fil: Zelarayán, Liliana Isabel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina
dc.journal.title
Zygote
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1017/S0967199414000240
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/zygote/article/role-of-arachidonic-acid-cascade-in-rhinella-arenarum-oocyte-maturation/29C7E3DAA700AC1317A38E0F911B036A
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