Artículo
Circulating tumor cells enumeration from the portal vein for risk stratification in early pancreatic cancer patients
Padillo Ruiz, Javier; Suarez, Gonzalo; Pereira, Sheila; Calero Castro, Francisco José; Tinoco, Jose; Marin, Luis; Bernal, Carmen; Cepeda Franco, Carmen; Alamo, Jose Maria; Almoguera, Francisco; Macher, Hada C.; Villanueva, Paula; García Fernandez, Francisco José; Gallego, Inmaculada; Romero, Manuel; Gomez Bravo, Miguel Angel; Denninghoff, Valeria Cecilia
; Serrano, María José
Fecha de publicación:
12/2021
Editorial:
MDPI
Revista:
Cancers
ISSN:
2072-6694
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background. Effective biomarkers are needed to enable personalized medicine for pancreatic cancer patients. This study analyzes the prognostic value, in early pancreatic cancer, of single circulating tumor cell (CTC) and CTC clusters from the central venous catheter (CVC) and portal blood (PV). Methods. In total, 7 mL of PV and CVC blood from 35 patients with early pancreatic cancer were analyzed. CTC were isolated using a positive immunomagnetic selection. The detection and identification of CTC were performed by immunocytochemistry (ICC) and were analyzed by Epi-fluorescence and confocal microscopy. Results. CTC and the clusters were detected both in PV and CVC. In both samples, the CTC number per cluster was higher in patients with grade three or poorly differentiated tumors (G3) than in patients with well (G1) or moderately (G2) differenti-ated. Patients with fewer than 185 CTC in PV exhibited a longer OS than patients with more than 185 CTC (24.5 vs. 10.0 months; p = 0.018). Similarly, patients with fewer than 15 clusters in PV showed a longer OS than patients with more than 15 clusters (19 vs. 10 months; p = 0.004). These significant correlations were not observed in CVC analyses. Conclusions. CTC presence in PV could be an important prognostic factor to predict poor prognosis in early pancreatic cancer. In addition, the number of clustered-CTC correlate to a tumor negative differentiation degree and, therefore, could be used as a diagnostic biomarker for pancreatic cancer.
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Licencia
Identificadores
Colecciones
Articulos(SEDE CENTRAL)
Articulos de SEDE CENTRAL
Articulos de SEDE CENTRAL
Citación
Padillo Ruiz, Javier; Suarez, Gonzalo; Pereira, Sheila; Calero Castro, Francisco José; Tinoco, Jose; et al.; Circulating tumor cells enumeration from the portal vein for risk stratification in early pancreatic cancer patients; MDPI; Cancers; 13; 24; 12-2021; 1-15
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