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dc.contributor.author De Burghgraeve, Tine
dc.contributor.author Kaptein, Suzanne J. F.
dc.contributor.author Ayala Nunez, Nilda V.
dc.contributor.author Mondotte, Juan Alberto
dc.contributor.author Pastorino, Boris
dc.contributor.author Printsevskaya, Svetlana S.
dc.contributor.author de Lamballerie, Xavier
dc.contributor.author Jacobs, Michael
dc.contributor.author Preobrazhenskaya, Maria
dc.contributor.author Gamarnik, Andrea Vanesa
dc.contributor.author Smit, Jolanda M.
dc.contributor.author Neyts, Johan
dc.date.available 2017-07-17T20:56:20Z
dc.date.issued 2012-05
dc.identifier.citation De Burghgraeve, Tine; Kaptein, Suzanne J. F.; Ayala Nunez, Nilda V.; Mondotte, Juan Alberto; Pastorino, Boris; et al.; An analogue of the antibiotic teicoplanin prevents flavivirus entry in vitro.; Public Library Of Science; Plos One; 7; 5; 5-2012; e37244
dc.identifier.issn 1932-6203
dc.identifier.uri http://hdl.handle.net/11336/20752
dc.description.abstract There is an urgent need for potent inhibitors of dengue virus (DENV) replication for the treatment and/or prophylaxis of infections with this virus. We here report on an aglycon analogue of the antibiotic teicoplanin (code name LCTA-949) that inhibits DENV-induced cytopathic effect (CPE) in a dose-dependent manner. Virus infection was completely inhibited at concentrations that had no adverse effect on the host cells. These findings were corroborated by quantification of viral RNA levels in culture supernatant. Antiviral activity was also observed against other flaviviruses such as the yellow fever virus and the tick-borne encephalitis virus (TBEV). In particular, potent antiviral activity was observed against TBEV. Time-of-drug-addition experiments indicated that LCTA-949 inhibits an early stage in the DENV replication cycle; however, a virucidal effect was excluded. This observation was corroborated by the fact that LCTA-949 lacks activity on DENV subgenomic replicon (that does not encode structural proteins) replication. Using a microsopy-based binding and fusion assay employing DiD-labeled viruses, it was shown that LCTA-949 targets the early stage (binding/entry) of the infection. Moreover, LCTA-949 efficiently inhibits infectivity of DENV particles pre-opsonized with antibodies, thus potentially also inhibiting antibody-dependent enhancement (ADE). In conclusion, LCTA-949 exerts in vitro activity against several flaviviruses and does so (as shown for DENV) by interfering with an early step in the viral replication cycle.
dc.format application/pdf
dc.language.iso eng
dc.publisher Public Library Of Science
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by/2.5/ar/
dc.subject antivirals
dc.subject dengue virus
dc.subject viral inhibition
dc.subject flavivirus
dc.subject.classification Virología
dc.subject.classification Ciencias Biológicas
dc.subject.classification CIENCIAS NATURALES Y EXACTAS
dc.title An analogue of the antibiotic teicoplanin prevents flavivirus entry in vitro.
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-06-12T20:59:37Z
dc.identifier.eissn 1932-6203
dc.journal.volume 7
dc.journal.number 5
dc.journal.pagination e37244
dc.journal.pais Estados Unidos
dc.journal.ciudad San Francisco
dc.description.fil Fil: De Burghgraeve, Tine. Katholikie Universiteit Leuven; Bélgica
dc.description.fil Fil: Kaptein, Suzanne J. F.. Katholikie Universiteit Leuven; Bélgica
dc.description.fil Fil: Ayala Nunez, Nilda V.. University of Groningen; Países Bajos
dc.description.fil Fil: Mondotte, Juan Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil Fil: Pastorino, Boris. Université de la Méditerranée; Francia
dc.description.fil Fil: Printsevskaya, Svetlana S.. Russian Academy of Medical Sciences; Rusia
dc.description.fil Fil: de Lamballerie, Xavier. Université de la Méditerranée; Francia
dc.description.fil Fil: Jacobs, Michael. Royal Free & University College Medical School; Reino Unido
dc.description.fil Fil: Preobrazhenskaya, Maria. Russian Academy of Medical Sciences; Rusia
dc.description.fil Fil: Gamarnik, Andrea Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina
dc.description.fil Fil: Smit, Jolanda M.. University of Groningen; Países Bajos
dc.description.fil Fil: Neyts, Johan. Katholikie Universiteit Leuven; Bélgica
dc.journal.title Plos One
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0037244
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/https://doi.org/10.1371/journal.pone.0037244


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info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution 2.5 Unported (CC BY 2.5)