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dc.contributor.author
Hasuoka, Paul Emir  
dc.contributor.author
Moyano, Mario Franco  
dc.contributor.author
Aragón, M. C.  
dc.contributor.author
Pacheco, Pablo Hugo  
dc.contributor.author
Fuentes, L. B  
dc.date.available
2023-08-04T15:26:35Z  
dc.date.issued
2019  
dc.identifier.citation
Effect of selenium as a protective agent during ischemia injury induced in transplant organs; XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo; San Luis; Argentina; 2020; 1-4  
dc.identifier.issn
1667-5746  
dc.identifier.uri
http://hdl.handle.net/11336/206996  
dc.description.abstract
Selenium is an essential element in human and animal metabolism, which is found as a constituent of selenoproteins. Selenium in selenocysteine form is integrated into the catalytic site of glutathione peroxidase (GPx), an antioxidant enzyme that protects the cells from the damage caused by reactive oxygen species (ROS). Oxidative stress is the imbalance between ROS and antioxidant defense systems, generating alterations of DNA, proteins, and lipid peroxidation. The imbalance occurs particularly during ischemia and lack of postmortem perfusion. This mechanism is of relevance in organs transplant, it affects the outcome during surgeries. The aim of this research was to evaluate the effect of selenium as a protective agent during ischemia injury induced postmortem in organs. Wistar rats (N=20) were administered via oral 75 μg/kg/day of seleno-methionine (Se-Met) during 7, 14, and 21 days. After sacrificed, the liver, heart, and kidney samples were collected at different postmortem intervals PMI (0-1-3-6-12 h). Total selenium concentration in organs was determined by Inductively Coupled Plasma Mass Spectrometry (ICP-MS). The administration of Se-Met produced a significant increase of selenium concentration in the liver (65%, P<0.001), heart (40%, P<0.01), and kidney (45%, P<0.05) respect to control. At 21 days, selenium showed significantly higher levels in kidney than control animals (3.75±0.24 vs.2.02±0.37 mg/kg, P<0.01). Oxidative stress was measured by malondialdehyde (MDA) liquid chromatography. The MDA decreased significantly in the heart (0.21±0.04 vs. 0.12±0.02 mmol/g) and the kidney (0.41±0.02 vs. 0.24±0.03 mmol/g) at one-hour PMI (P<0.01). Organs removed at different PMI intervals showed lower production of MDA compared to the control group (P<0.05). Se-Met decreased significantly oxidative stress in transplant organs from 1 to 12 h PMI. The results suggest that selenium is a protective agent that improves transplant organs‟ survival and the outcome of transplant surgeries. Future studies will be focused on the specific activity of GPx and selenium in transplant organs.  
dc.format
application/pdf  
dc.language.iso
eng  
dc.publisher
Sociedad de Biología de Cuyo  
dc.rights
info:eu-repo/semantics/restrictedAccess  
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/  
dc.subject
Selenio  
dc.subject
Ratas  
dc.subject
Isquemia  
dc.subject
Organo  
dc.subject.classification
Otras Ciencias Biológicas  
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Ciencias Biológicas  
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CIENCIAS NATURALES Y EXACTAS  
dc.title
Effect of selenium as a protective agent during ischemia injury induced in transplant organs  
dc.type
info:eu-repo/semantics/publishedVersion  
dc.type
info:eu-repo/semantics/conferenceObject  
dc.type
info:ar-repo/semantics/documento de conferencia  
dc.date.updated
2022-11-09T18:37:48Z  
dc.journal.volume
44  
dc.journal.number
A45  
dc.journal.pagination
1-4  
dc.journal.pais
Argentina  
dc.journal.ciudad
San Luis  
dc.description.fil
Fil: Hasuoka, Paul Emir. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina  
dc.description.fil
Fil: Moyano, Mario Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina  
dc.description.fil
Fil: Aragón, M. C.. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina  
dc.description.fil
Fil: Pacheco, Pablo Hugo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina  
dc.description.fil
Fil: Fuentes, L. B. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina  
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://sbcuyo.org.ar/wp-content/uploads/2021/07/2020-XXXVII-Scientific-Meeting-of-the-Cuyo-Biology-Society2.pdf  
dc.conicet.rol
Autor  
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Autor  
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Autor  
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Autor  
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Autor  
dc.coverage
Nacional  
dc.type.subtype
Reunión  
dc.description.nombreEvento
XXXVII Reunión Científica Anual de la Sociedad de Biología de Cuyo  
dc.date.evento
2020-12-05  
dc.description.ciudadEvento
San Luis  
dc.description.paisEvento
Argentina  
dc.type.publicacion
Journal  
dc.description.institucionOrganizadora
Sociedad de Biología de Cuyo  
dc.source.revista
Biocell  
dc.date.eventoHasta
2020-12-06  
dc.type
Reunión