Mostrar el registro sencillo del ítem

dc.contributor.author Acevedo, Raúl Maximiliano
dc.contributor.author Ruiz, Oscar Adolfo
dc.contributor.author Sansberro, Pedro Alfonso
dc.date.available 2017-07-14T18:36:14Z
dc.date.issued 2016-10
dc.identifier.citation Acevedo, Raúl Maximiliano; Ruiz, Oscar Adolfo; Sansberro, Pedro Alfonso; Transcriptome expression profiling in response to drought stress in Ilex paraguariensis leaves; Southern Cross Publ; Plant Omics; 9; 5; 10-2016; 334-343
dc.identifier.issn 1836-0661
dc.identifier.uri http://hdl.handle.net/11336/20660
dc.description.abstract Ilex paraguariensis St. Hil. is recognized as a shade-tolerant tree, cultivated in north-eastern Argentina, south-eastern Brazil, and Paraguay. Its leaves and young shoots are industrialised and; hence, being used to prepare a traditional tea-like infusion (mate-tea) with various medicinal benefits. Under the prevailing agro-climatic conditions in NE Argentina and with the occurrence of high temperatures and scarce rains during spring or summer, I. paraguariensis plants are subjected to water stress, reducing their yield. Differential Display was used to compare differences in gene expression between stressed and unstressed (control) potted plants. We found 52 differentially expressed transcripts (DETs), analysing banding patterns of Differential Display polyacrylamide gels, in which 38 DETs were induced, and 14 DETs were repressed in response to drought. Sequence comparisons revealed that 19% of the fragments showed no homologies, whereas 40.5 % of the total number of fragments had strong similarities with database entries of known functions. The other DETs belonged to unclassified proteins or genes with unknown functions. Interestingly, 12% of the DNA sequences were related to signal transduction, and 2% of the sequences were identified as transcription factors. Approximately, 12% of the annotated sequences were involved in stress tolerance and metal homeostasis, while 7% of the DETs were associated with photosynthesis. Other groups, representing 12%, included those involved in secondary metabolism, lignin biosynthesis, cell walls and RNA stabilization/chaperone activity. When integrated with physiological data, our results suggest that the transcriptional activity of some of the studied genes could be related to the degree of drought tolerance/susceptibility of I. paraguariensis.
dc.format application/pdf
dc.language.iso eng
dc.publisher Southern Cross Publ
dc.rights info:eu-repo/semantics/openAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject abiotic stress
dc.subject gene expression
dc.subject mRNA differential display
dc.subject Ilex paraguariensis
dc.subject.classification Biotecnología Agrícola y Biotecnología Alimentaria
dc.subject.classification Biotecnología Agropecuaria
dc.subject.classification CIENCIAS AGRÍCOLAS
dc.title Transcriptome expression profiling in response to drought stress in Ilex paraguariensis leaves
dc.type info:eu-repo/semantics/article
dc.type info:ar-repo/semantics/artículo
dc.type info:eu-repo/semantics/publishedVersion
dc.date.updated 2017-07-13T19:09:51Z
dc.identifier.eissn 1836-3644
dc.journal.volume 9
dc.journal.number 5
dc.journal.pagination 334-343
dc.journal.pais Australia
dc.description.fil Fil: Acevedo, Raúl Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.description.fil Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
dc.description.fil Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.journal.title Plant Omics
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.21475/poj.09.05.16.pne185
dc.relation.alternativeid info:eu-repo/semantics/altIdentifier/url/http://www.pomics.com/sansberro_9_5_2016_334_343.pdf


Archivos asociados

Aparece en la(s) siguiente(s) colección(ones)

Mostrar el registro sencillo del ítem

info:eu-repo/semantics/openAccess Excepto donde se diga explícitamente, este item se publica bajo la siguiente descripción: Creative Commons Attribution-NonCommercial-ShareAlike 2.5 Unported (CC BY-NC-SA 2.5)