Mostrar el registro sencillo del ítem

dc.contributor.author
Acevedo, Raúl Maximiliano
dc.contributor.author
Ruiz, Oscar Adolfo
dc.contributor.author
Sansberro, Pedro Alfonso
dc.date.available
2017-07-14T18:36:14Z
dc.date.issued
2016-10
dc.identifier.citation
Acevedo, Raúl Maximiliano; Ruiz, Oscar Adolfo; Sansberro, Pedro Alfonso; Transcriptome expression profiling in response to drought stress in Ilex paraguariensis leaves; Southern Cross Publ; Plant Omics; 9; 5; 10-2016; 334-343
dc.identifier.issn
1836-0661
dc.identifier.uri
http://hdl.handle.net/11336/20660
dc.description.abstract
Ilex paraguariensis St. Hil. is recognized as a shade-tolerant tree, cultivated in north-eastern Argentina, south-eastern Brazil, and Paraguay. Its leaves and young shoots are industrialised and; hence, being used to prepare a traditional tea-like infusion (mate-tea) with various medicinal benefits. Under the prevailing agro-climatic conditions in NE Argentina and with the occurrence of high temperatures and scarce rains during spring or summer, I. paraguariensis plants are subjected to water stress, reducing their yield. Differential Display was used to compare differences in gene expression between stressed and unstressed (control) potted plants. We found 52 differentially expressed transcripts (DETs), analysing banding patterns of Differential Display polyacrylamide gels, in which 38 DETs were induced, and 14 DETs were repressed in response to drought. Sequence comparisons revealed that 19% of the fragments showed no homologies, whereas 40.5 % of the total number of fragments had strong similarities with database entries of known functions. The other DETs belonged to unclassified proteins or genes with unknown functions. Interestingly, 12% of the DNA sequences were related to signal transduction, and 2% of the sequences were identified as transcription factors. Approximately, 12% of the annotated sequences were involved in stress tolerance and metal homeostasis, while 7% of the DETs were associated with photosynthesis. Other groups, representing 12%, included those involved in secondary metabolism, lignin biosynthesis, cell walls and RNA stabilization/chaperone activity. When integrated with physiological data, our results suggest that the transcriptional activity of some of the studied genes could be related to the degree of drought tolerance/susceptibility of I. paraguariensis.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Southern Cross Publ
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Abiotic Stress
dc.subject
Gene Expression
dc.subject
Mrna Differential Display
dc.subject
Ilex Paraguariensis
dc.subject.classification
Biotecnología Agrícola y Biotecnología Alimentaria
dc.subject.classification
Biotecnología Agropecuaria
dc.subject.classification
CIENCIAS AGRÍCOLAS
dc.title
Transcriptome expression profiling in response to drought stress in Ilex paraguariensis leaves
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-13T19:09:51Z
dc.identifier.eissn
1836-3644
dc.journal.volume
9
dc.journal.number
5
dc.journal.pagination
334-343
dc.journal.pais
Australia
dc.description.fil
Fil: Acevedo, Raúl Maximiliano. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.description.fil
Fil: Ruiz, Oscar Adolfo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas; Argentina
dc.description.fil
Fil: Sansberro, Pedro Alfonso. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina
dc.journal.title
Plant Omics
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.21475/poj.09.05.16.pne185
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.pomics.com/sansberro_9_5_2016_334_343.pdf


Archivos asociados

 

Comunidades y colecciones

Mostrar el registro sencillo del ítem