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dc.contributor.author
Edwards, Anne
dc.contributor.author
Frederix, Marijke
dc.contributor.author
Wisniewski-Dyé, Florence
dc.contributor.author
Jones, Jacob
dc.contributor.author
Zorreguieta, Angeles
dc.contributor.author
Downie, Allan
dc.date.available
2017-07-14T17:58:00Z
dc.date.issued
2009-03
dc.identifier.citation
Edwards, Anne; Frederix, Marijke; Wisniewski-Dyé, Florence; Jones, Jacob; Zorreguieta, Angeles; et al.; The cin and rai quorum-sensing regulatory systems in Rhizobium leguminosarum are coordinated by ExpR and CinS, a small regulatory protein coexpressed with CinI.; American Society for Microbiology; Journal Of Bacteriology; 191; 9; 3-2009; 3059-3067
dc.identifier.issn
0021-9193
dc.identifier.uri
http://hdl.handle.net/11336/20623
dc.description.abstract
To understand how the Rhizobium leguminosarum raiI-raiR quorum-sensing system is regulated, we identified mutants with decreased levels of RaiI-made N-acyl homoserine lactones (AHLs). A LuxR-type regulator, ExpR, is required for raiR expression, and RaiR is required to induce raiI. Since raiR (and raiI) expression is also reduced in cinI and cinR quorum-sensing mutants, we thought CinI-made AHLs may activate ExpR to induce raiR. However, added CinI-made AHLs did not induce raiR expression in a cinI mutant. The reduced raiR expression in cinI and cinR mutants was due to lack of expression of cinS immediately downstream of cinI. cinS encodes a 67-residue protein, translationally coupled to CinI, and cinS acts downstream of expR for raiR induction. Cloned cinS in R. leguminosarum caused an unusual collapse of colony structure, and this was delayed by mutation of expR. The phenotype looked like a loss of exopolysaccharide (EPS) integrity; mutations in cinI, cinR, cinS, and expR all reduced expression of plyB, encoding an EPS glycanase, and mutation of plyB abolished the effect of cloned cinS on colony morphology. We conclude that CinS and ExpR act to increase PlyB levels, thereby influencing the bacterial surface. CinS is conserved in other rhizobia, including Rhizobium etli; the previously observed effect of cinI and cinR mutations decreasing swarming in that strain is primarily due to a lack of CinS rather than a lack of CinI-made AHL. We conclude that CinS mediates quorum-sensing regulation because it is coregulated with an AHL synthase and demonstrate that its regulatory effects can occur in the absence of AHLs.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
American Society for Microbiology
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Quorum
dc.subject
Rhizobium
dc.subject
Glycanase
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Regulation
dc.subject.classification
Bioquímica y Biología Molecular
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
The cin and rai quorum-sensing regulatory systems in Rhizobium leguminosarum are coordinated by ExpR and CinS, a small regulatory protein coexpressed with CinI.
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-11T19:28:35Z
dc.identifier.eissn
1098-5530
dc.journal.volume
191
dc.journal.number
9
dc.journal.pagination
3059-3067
dc.journal.pais
Estados Unidos
dc.journal.ciudad
Washington
dc.description.fil
Fil: Edwards, Anne. John Innes Institute; Reino Unido
dc.description.fil
Fil: Frederix, Marijke. John Innes Institute; Reino Unido
dc.description.fil
Fil: Wisniewski-Dyé, Florence. Université Claude Bernard Lyon 1; Francia
dc.description.fil
Fil: Jones, Jacob. John Innes Institute; Reino Unido
dc.description.fil
Fil: Zorreguieta, Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Biológica; Argentina
dc.description.fil
Fil: Downie, Allan. John Innes Institute; Reino Unido
dc.journal.title
Journal Of Bacteriology
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://jb.asm.org/content/191/9/3059.long
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1128/JB.01650-08
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