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dc.contributor.author
Albarracín, Leonardo Miguel
dc.contributor.author
Ortiz Moyano, Francisco Ramiro
dc.contributor.author
Vargas, Juan Martin
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Andrade, Bruno G. N.
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Cortez Zamar, Juan Marcos
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Dentice Maidana, Stefania
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Fukuyama, Kohtaro
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Kurata, Shoichiro
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Jure, Maria Angela
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Kitazawa, Haruki
dc.contributor.author
Villena, Julio Cesar
dc.date.available
2023-07-31T11:13:23Z
dc.date.issued
2022-07
dc.identifier.citation
Albarracín, Leonardo Miguel; Ortiz Moyano, Francisco Ramiro; Vargas, Juan Martin; Andrade, Bruno G. N.; Cortez Zamar, Juan Marcos; et al.; Genomic and immunological characterization of hypermucoviscous carbapenem-resistant klebsiella pneumoniae ST25 isolates from northwest Argentina; MDPI; International Journal of Molecular Sciences; 23; 13; 7-2022; 1-20
dc.identifier.issn
1661-6596
dc.identifier.uri
http://hdl.handle.net/11336/206088
dc.description.abstract
In recent years, an increase in the prevalence hypermucoviscous carbapenem-resistant Klebsiella pneumoniae with sequence type 25 (ST25) was detected in hospitals of Tucuman (Northwest Argentina). In this work, the virulence and the innate immune response to two K. pneumoniae ST25 strains (LABACER 01 and LABACER 27) were evaluated in a murine model after a respiratory challenge. In addition, comparative genomics was performed with K. pneumoniae LABACER01 and LABACER27 to analyze genes associated with virulence. Both LABACER01 and LABACER27 were detected in the lungs of infected mice two days after the nasal challenge, with LABACER01 counts significantly higher than those of LABACER27. Only LABACER01 was detected in hemocultures. Lactate dehydrogenase (LDH) and albumin levels in bronchoalveolar lavage (BAL) samples were significantly higher in mice challenged with LABACER01 than in LABACER27-infected animals, indicating greater lung tissue damage. Both strains increased the levels of neutrophils, macrophages, TNF-α, IL-1β, IL-6, KC, MCP-1, IFN-γ, and IL-17 in the respiratory tract and blood, with the effect of LABACER01 more marked than that of LABACER27. In contrast, LABACER27 induced higher levels of IL-10 in the respiratory tract than LABACER01. Genomic analysis revealed that K. pneumoniae LABACER01 and LABACER27 possess virulence factors found in other strains that have been shown to be hypervirulent, including genes required for enterobactin (entABCDEF) and salmochelin (iroDE) biosynthesis. In both strains, the genes of toxin–antitoxin systems, as well as regulators of the expression of virulence factors and adhesion genes were also detected. Studies on the genetic potential of multiresistant K. pneumoniae strains as well as their cellular and molecular interactions with the host are of fundamental importance to assess the association of certain virulence factors with the intensity of the inflammatory response. In this sense, this work explored the virulence profile based on genomic and in vivo studies of hypermucoviscous carbapenem-resistant K. pneumoniae ST25 strains, expanding the knowledge of the biology of the emerging ST25 clone in Argentina.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
MDPI
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by/2.5/ar/
dc.subject
CARBAPENEM RESISTANT
dc.subject
GENOMIC
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HYPERMUCOVISCOUS
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KLEBSIELLA PNEUMONIAE
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RESPIRATORY INFECTION
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SEQUENCE TYPE 25
dc.subject.classification
Biología Celular, Microbiología
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Ciencias Biológicas
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CIENCIAS NATURALES Y EXACTAS
dc.title
Genomic and immunological characterization of hypermucoviscous carbapenem-resistant klebsiella pneumoniae ST25 isolates from northwest Argentina
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2023-07-07T19:02:06Z
dc.identifier.eissn
1422-0067
dc.journal.volume
23
dc.journal.number
13
dc.journal.pagination
1-20
dc.journal.pais
Suiza
dc.journal.ciudad
Basel
dc.description.fil
Fil: Albarracín, Leonardo Miguel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Ciencias Exactas y Tecnología; Argentina
dc.description.fil
Fil: Ortiz Moyano, Francisco Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.description.fil
Fil: Vargas, Juan Martin. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Andrade, Bruno G. N.. Munster Technological University; Irlanda
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Fil: Cortez Zamar, Juan Marcos. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
dc.description.fil
Fil: Dentice Maidana, Stefania. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
dc.description.fil
Fil: Fukuyama, Kohtaro. Tohoku University; Japón
dc.description.fil
Fil: Kurata, Shoichiro. Tohoku University; Japón
dc.description.fil
Fil: Jure, Maria Angela. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia. Instituto de Microbiología; Argentina
dc.description.fil
Fil: Kitazawa, Haruki. Tohoku University; Japón
dc.description.fil
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
dc.journal.title
International Journal of Molecular Sciences
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.3390/ijms23137361
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/1422-0067/23/13/7361
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