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dc.contributor.author
Moreno, Natalia Cecilia
dc.contributor.author
Las Peñas, Maria Laura
dc.contributor.author
Bernardello, Gabriel Luis Mario
dc.contributor.author
Roitman, Germán
dc.date.available
2017-07-14T17:39:54Z
dc.date.issued
2009-12
dc.identifier.citation
Moreno, Natalia Cecilia; Las Peñas, Maria Laura; Bernardello, Gabriel Luis Mario; Roitman, Germán; Cytogenetic studies in Herbertia Sw. (Iridaceae); Univ Florence Botany Inst; Caryologia; 60; 4; 12-2009; 37-42
dc.identifier.issn
0008-7114
dc.identifier.uri
http://hdl.handle.net/11336/20607
dc.description.abstract
Cnventional Feulgen staining, CMA/DAPI banding and FISH with 18-5.8-26S rDNA probe were used to determine chromosome numbers, karyotypes and physical position of GC or AT rich heterochromatin and rDNA sites in three South American species of Herbertia: H. lahue subsp. amoena, H. quareimana, and H. darwinii. The basic number of the genus is x=7, but the analyzed taxa have different ploidy levels. The chromosome number was 2n=14 for H. darwinii and 2n=28 for H. quareimana. The two populations studied of H. lahue subsp. amoena did result in: 2n=42 (Gualeguaychu) and 56 (Palma de Castillo). Chromosome size varied between 1.60 and 5 µm. The karyotypes did give with a total of 8 metacentric (m) + 6 submetacentric (sm) chromosomes for H. darwinii and 20 m + 8 sm chromosomes for H. quareimana. The two populations of H. lahue subsp. amoena showed with 26 m + 16 sm chromosomes and 36 m + 20 sm chromosomes. H. darwinii had a bimodal karyotype. H. darwinii showed one satellited pair whereas the remaining species two (always in short m chromosome arms). Moreover, the 2n=56 population of H. lahue subsp. amoena one satellite on the long arms. No CMA- /DAPI+ bands were detected in any species, but CMA+/DAPI– bands associated with nucleolar organizing regions (NOR) were found in all cases. The locations of the 18-5.8-26S rDNA sites in all species coincided with CMA+/DAPI- bands. Our data suggest that karyotypic diversification in Herbertia included changes in chromosome number, symmetry, and number and position of NORs regions.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Univ Florence Botany Inst
dc.rights
info:eu-repo/semantics/restrictedAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cma/Dapi Banding
dc.subject
Fish
dc.subject
Herbetia
dc.subject
Karyotype
dc.subject
Polyploidy
dc.subject
Somatic Chromosomes
dc.subject.classification
Genética y Herencia
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Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Cytogenetic studies in Herbertia Sw. (Iridaceae)
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-13T18:26:32Z
dc.identifier.eissn
2165-5391
dc.journal.volume
60
dc.journal.number
4
dc.journal.pagination
37-42
dc.journal.pais
Italia
dc.description.fil
Fil: Moreno, Natalia Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
dc.description.fil
Fil: Las Peñas, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
dc.description.fil
Fil: Bernardello, Gabriel Luis Mario. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina
dc.description.fil
Fil: Roitman, Germán. Universidad de Buenos Aires. Facultad de Agronomia; Argentina
dc.journal.title
Caryologia
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/pdf/10.1080/00087114.2004.10589664
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/00087114.2004.10589664


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