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dc.contributor.author
Ortiz, María Eugenia
dc.contributor.author
Bühler, Marta Ines
dc.contributor.author
Zelarayán, Liliana Isabel
dc.date.available
2017-07-14T16:31:19Z
dc.date.issued
2013-02
dc.identifier.citation
Ortiz, María Eugenia; Bühler, Marta Ines; Zelarayán, Liliana Isabel; Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation; Cambridge University Press; Zygote; 22; 4; 2-2013; 440-445
dc.identifier.issn
0967-1994
dc.identifier.uri
http://hdl.handle.net/11336/20540
dc.description.abstract
In Rhinella arenarum, progesterone is the physiological nuclear maturation inducer that interacts with oocyte surface and starts a cascade of events leading to germinal vesicle breakdown (GVBD). Polyunsaturated fatty acids and their metabolites produced through cyclooxygenase (COX) and lipoxygenase (LOX) pathways play an important role in reproductive processes. In amphibians, the role of arachidonic acid (AA) metabolites in P4-induced oocyte maturation has not been clarified yet. In this work we studied the participation of enzymes involved in AA metabolism ?phospholipase A2 (PLA2), COX and LOX- in Rhinella arenarum oocyte maturation. PLA2 activation induced maturation in Rhinella arenarum oocytes in a dose-dependent manner. Oocytes treated with melittin showed highest response (786% GVBD) with 0.08 µM. In follicles, PLA2 activation did not significantly induce maturation at the assayed doses (123% GVBD). PLA2 inhibition with quinacrine prevented melittin-induced GVBD in a dose-dependent manner; however, PLA2 inactivation did not affect P4-induced maturation. This suggests that PLA2 is not the only phospholipase involved in P4-induced maturation in this species. P4-induced oocyte maturation was inhibited by COX inhibitors, indomethacin and rofecoxib (653% and 633% GVBD, respectively), although it was never blocked by them. Follicles showed a similar response to these inhibitors. Participation of LOX metabolites in maturation seems to be correlated with seasonal variations in ovarian response to P4. During the February-June period (low P4 response), LOX inhibition by nordihydroguaiaretic acid (NDGA) or lysine clonixinate (LC) increased maturation up to 70%. During the July-January period (high P4 response), LOX inhibition had no effect on hormone-induced maturation.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Cambridge University Press
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Cyclooxigenase
dc.subject
Lipooxigenase
dc.subject
Oocyte Maturation
dc.subject
Phospholipase A2
dc.subject
Rhinella Arenarum
dc.subject.classification
Bioquímica y Biología Molecular
dc.subject.classification
Ciencias Biológicas
dc.subject.classification
CIENCIAS NATURALES Y EXACTAS
dc.title
Involvement of PLA2, COX and LOX in Rhinella arenarum oocyte maturation
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-03T20:14:44Z
dc.identifier.eissn
1469-8730
dc.journal.volume
22
dc.journal.number
4
dc.journal.pagination
440-445
dc.journal.pais
Reino Unido
dc.journal.ciudad
Cambridge
dc.description.fil
Fil: Ortiz, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
dc.description.fil
Fil: Bühler, Marta Ines. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina
dc.description.fil
Fil: Zelarayán, Liliana Isabel. Universidad Nacional de Tucuman. Facultad de Bioquímica, Química y Farmacia. Instituto de Biología; Argentina
dc.journal.title
Zygote
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.cambridge.org/core/journals/zygote/article/involvement-of-pla2-cox-and-lox-in-rhinella-arenarum-oocyte-maturation/82FB8AF0C94B4D3110A4A9D1683FA998
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1017/S096719941200069X
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