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dc.contributor.author
Prochetto, Santiago
dc.contributor.author
Gonzalez, Veronica Doris Guadalupe
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Garcia, Valeria Soledad
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Vanasco, Norma Bibiana
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Lottersberger, Javier
dc.contributor.author
Gugliotta, Luis Marcelino
dc.date.available
2017-07-14T13:02:36Z
dc.date.issued
2017-02
dc.identifier.citation
Prochetto, Santiago; Gonzalez, Veronica Doris Guadalupe; Garcia, Valeria Soledad; Vanasco, Norma Bibiana; Lottersberger, Javier; et al.; Latex Particles Sensitized with Proteins of Leptospira interrogans for Application in Immunoagglutination Assays; Taylor & Francis As; International Journal Of Polymeric Materials; 2-2017
dc.identifier.issn
0091-4037
dc.identifier.uri
http://hdl.handle.net/11336/20488
dc.description.abstract
Physical adsorption and chemical coupling of recombinant proteins of Leptospira interrogans onto polystyrene and core-shell carboxylated particles were, respectively, investigated with the aim of producing latex?protein complexes to be used in immunoagglutination assays able to detect the leptospirosis disease in either humans or animals. To this effect, a protein lysate of crude extracts was evaluated, and sensitizations were carried out at different pHs, with the antigenic proteins approach to the particle surface favored at pH close to their isoelectric point. In the covalent coupling experiments, high fractions of proteins were chemically bound to carboxyl groups on the particle surface and higher densities of linked proteins were obtained for particles exhibiting greater carboxyl group densities. The produced latex?protein complexes were tested in immunoagglutination assays, by turbidimetry and a visual method, from a panel that included positive and negative bovine, canine, and human sera. The area under receiver operating characteristic curves was used as an index of accuracy. The complexes obtained by covalent coupling of proteins on the latex of higher density of carboxyl groups allowed an acceptable discrimination between the studied positive and negative sera.
dc.format
application/pdf
dc.language.iso
eng
dc.publisher
Taylor & Francis As
dc.rights
info:eu-repo/semantics/openAccess
dc.rights.uri
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.subject
Immunoagglutination
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Latex-Protein Complex
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Sensitized Particles
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Leptospirosis
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Otras Ingeniería de los Materiales
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Ingeniería de los Materiales
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INGENIERÍAS Y TECNOLOGÍAS
dc.title
Latex Particles Sensitized with Proteins of Leptospira interrogans for Application in Immunoagglutination Assays
dc.type
info:eu-repo/semantics/article
dc.type
info:ar-repo/semantics/artículo
dc.type
info:eu-repo/semantics/publishedVersion
dc.date.updated
2017-07-10T13:10:31Z
dc.journal.pais
Reino Unido
dc.description.fil
Fil: Prochetto, Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Gonzalez, Veronica Doris Guadalupe. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
dc.description.fil
Fil: Garcia, Valeria Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.description.fil
Fil: Vanasco, Norma Bibiana. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
dc.description.fil
Fil: Lottersberger, Javier. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
dc.description.fil
Fil: Gugliotta, Luis Marcelino. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Desarrollo Tecnológico para la Industria Química. Universidad Nacional del Litoral. Instituto de Desarrollo Tecnológico para la Industria Química; Argentina
dc.journal.title
International Journal Of Polymeric Materials
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/url/https://www.tandfonline.com/doi/full/10.1080/00914037.2016.1276058
dc.relation.alternativeid
info:eu-repo/semantics/altIdentifier/doi/http://dx.doi.org/10.1080/00914037.2016.1276058
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