Artículo
Pleiotropic actions of melatonin in testicular peritubular myoid cells of immature Syrian hamsters
Riviere, Eugenia
; Rossi, Soledad Paola
; Tavalieri, Yamil Ezequiel; Muñoz de Toro, Monica Milagros; Calandra, Ricardo Saul
; Mayerhofer, Artur; Matzkin, Maria Eugenia
; Frungieri, Monica Beatriz
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Fecha de publicación:
10/2022
Editorial:
Elsevier Science
Revista:
Biochimica et Biophysica Acta - General Subjects
ISSN:
0304-4165
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Background: Peritubular myoid cells are emerging as key regulators of testicular function in adulthood. However, little is known about the role of testicular peritubular myoid cells (TPMCs) in the development of the male gonad. We found that, compared to testes of young adult hamsters, gonads of 21 day-old animals show increased melatonin concentration, seminiferous tubular wall thickening and a heterogeneous packaging of its collagen fibers thus raising the question whether melatonin may be involved in the regulation of TPMCs. Methods: We established primary cultures of TPMCs from immature hamsters (ihaTPMCs), which we found express melatonergic receptors. Results: Exogeneous melatonin decreased the levels of inflammatory markers (NLRP3 inflammasome, IL1β) but increased the expression of cyclooxygenase 2 (COX2, key enzyme mediating prostaglandin synthesis) and of the glial cell line-derived neurotrophic factor (GDNF) in ihaTPMCs. Melatonin also stimulated ihaTPMCs proliferation and the expression of extracellular matrix proteins such as collagen type I and IV. Furthermore, collagen gel contraction assays revealed an enhanced ability of ihaTPMCs to contract in the presence of melatonin. Conclusion: Melatonin regulates immune and inflammatory functions as well as contractile phenotype of the peritubular wall in the hamster testis. General significance: If transferable to the in vivo situation, melatonin-dependent induction of ihaTPMCs to produce factors known to exert paracrine effects in other somatic cell populations of the gonad suggests that the influence of melatonin may go beyond the peritubular wall and indicates its contribution to testicular development and the establishment of a normal and sustainable spermatogenesis.
Palabras clave:
CONTRACTILITY
,
HAMSTER
,
IMMUNOLOGY
,
MELATONIN
,
PERITUBULAR MYOID CELLS
,
TESTIS
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Colecciones
Articulos(IBYME)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos de INST.DE BIOLOGIA Y MEDICINA EXPERIMENTAL (I)
Articulos(ISAL)
Articulos de INSTITUTO DE SALUD Y AMBIENTE DEL LITORAL
Articulos de INSTITUTO DE SALUD Y AMBIENTE DEL LITORAL
Citación
Riviere, Eugenia; Rossi, Soledad Paola; Tavalieri, Yamil Ezequiel; Muñoz de Toro, Monica Milagros; Calandra, Ricardo Saul; et al.; Pleiotropic actions of melatonin in testicular peritubular myoid cells of immature Syrian hamsters; Elsevier Science; Biochimica et Biophysica Acta - General Subjects; 1866; 10; 10-2022; 1-13
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