Artículo
Ezrin and CD44 participate in the internalization process of Coxiella burnetii into non-phagocytic cells
Distel, Jesús Sebastián
; Ortiz Flores, Rodolfo Matias
; Bienvenu, Arthur; Aguilera, Milton Osmar
; Bonazzi, Matteo; Abraham, Carlos Walter
Fecha de publicación:
07/2022
Editorial:
Journal of the Serbian Chemical Society
Revista:
Biology Of The Cell
ISSN:
0248-4900
Idioma:
Inglés
Tipo de recurso:
Artículo publicado
Clasificación temática:
Resumen
Ezrin protein is involved in the interaction of actin cytoskeleton with membrane receptors such as CD44. It regulates plasma membrane dynamics and intracellular signaling. Coxiella burnetii, the etiologic agent of Q fever, is internalized into host cell through a poorly characterized molecular mechanism. Here we analyzed the role of ezrin and CD44 in the C. burnetii internalization by HeLa cells. The knockdown of ezrin and CD44 inhibited the bacterial uptake. Interestingly, at early stages of C. burnetii internalization, ezrin was recruited to the cell membrane fraction and phosphorylated. Moreover, the overexpression of non-phosphorylatable and phosphomimetic ezrin mutants decreased and increased the bacterial entry, respectively. A decrease in the internalization of C. burnetii was observed by the overexpression of CD44 truncated forms containing the intracellular or the extracellular domains. Interestingly, the CD44 mutant was unable to interact with ERM proteins decreased the bacterial internalization. These findings demonstrate the participation of ezrin in the internalization process of C. burnetii in non-phagocytic cells. Additionally, we present evidence that CD44 receptor would be involved in that process.
Palabras clave:
CD44
,
COXIELLA BURNETII
,
EZRIN
,
PHAGOCYTOSIS
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Licencia
Identificadores
Colecciones
Articulos(IHEM)
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Articulos de INST. HISTOLOGIA Y EMBRIOLOGIA DE MEND DR.M.BURGOS
Citación
Distel, Jesús Sebastián; Ortiz Flores, Rodolfo Matias; Bienvenu, Arthur; Aguilera, Milton Osmar; Bonazzi, Matteo; et al.; Ezrin and CD44 participate in the internalization process of Coxiella burnetii into non-phagocytic cells; Journal of the Serbian Chemical Society; Biology Of The Cell; 114; 9; 7-2022; 237-253
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